UBE2I Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P63279

Core Product Specifications and Parameters

Biological Background: UBE2I Function and Localization

• UBE2I encodes the SUMO-conjugating enzyme UBC9 (also designated p18, ubiquitin carrier protein 9, and ubiquitin-conjugating enzyme E2 I), a central E2 enzyme in the SUMOylation cascade. It accepts SUMO1–4 and SUMO5 from the E1 complex and catalyzes their covalent ligation to target proteins, often with the assistance of E3 ligases such as RANBP2, CBX4, and ZNF451.
• UBC9 is essential for nuclear architecture and chromosome segregation, playing a pivotal role in mitosis and cell division.
• It sumoylates a diverse array of substrates, including p53/TP53 at Lys-386, ERCC6 for transcription-coupled nucleotide excision repair, SHMT1 at Lys-38/39 to direct its nuclear import, and TYMS and DHFR. Related references: PMID:26620705, PMID:17446168, PMID:18067453
• The protein is predominantly nuclear but also localizes to the cytoplasm with perinuclear enrichment.
• UBE2I is widely expressed across tissues, including heart, skeletal muscle, pancreas, kidney, liver, lung, placenta, brain, testis, and thymus.
• Post-translational modifications include acetylation and phosphorylation; it features ATP- and nucleotide-binding motifs and forms isopeptide bonds during SUMO conjugation.
• UBC9 is implicated in host-virus interactions, reflecting its broad functional repertoire.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–100 of human UBE2I; the resulting monoclonal antibody targets the N‑terminal region and may detect both unmodified and sumoylated forms.
• For Western blotting, the predicted molecular weight is approximately 18 kDa; use a high‑resolution gel system to ensure distinct band separation in the low molecular weight range and validate with appropriate controls.
• The antibody is suitable for IHC‑P; consider standard antigen retrieval methods and optimize staining conditions for your tissue type.
• Cross‑reactivity is confirmed for human, mouse, and rat; verify species‑specific reactivity in the intended experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this UBE2I (UBC9) monoclonal antibody as part of a comprehensive portfolio of IVD raw materials, supporting researchers and diagnostic developers in sumoylation, cancer biology, and cell cycle studies. Our integrated supply chain ensures reliable access to validated antibody pairs, bulk reagents, and rare-target sourcing, streamlining your progression from concept to clinical application.