UBE2F Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q969M7

Core Product Specifications and Parameters

Biological Background: UBE2F Function and Localization

• UBE2F, also known as NEDD8-conjugating enzyme 2 (NCE2), accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to target proteins. Related references: PMID:19250909, PMID:23201271
• Together with the E3 ubiquitin ligase RNF7/RBX2, UBE2F specifically neddylates cullin-5 (CUL5). Related references: PMID:19250909, PMID:23201271, PMID:23300442
• UBE2F does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Related references: PMID:19250909, PMID:23201271
• UBE2F mediates neddylation of the CUL9-RBX1 complex. Related references: PMID:38605244
• Upon HIV-1 infection, UBE2F participates in Vif-mediated ubiquitination and degradation of APOBEC3G by neddylating CUL5.
• Widely expressed at the protein level across tissues.
• The protein belongs to the ubiquitin-conjugating enzyme family and features ATP-binding and nucleotide-binding motifs, consistent with its transferase role in the Ubl conjugation pathway.
• Post-translational modifications include acetylation and alternative splicing events.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-185 of human UBE2F, which corresponds to the full-length protein. This design likely enables recognition of native and denatured forms.
• For Western blot, a band around 21 kDa is expected. Ensure proper protein extraction and loading controls.
• IHC-P and IF/ICC may require antigen retrieval and optimized permeabilization, respectively. Validate with appropriate positive and negative tissue/cell controls.
• The antibody cross-reacts with mouse and rat, making it suitable for preclinical models. However, sequence alignment in the target epitope regions should be verified for your specific experimental setup.
• As a polyclonal antibody, lot-to-lot consistency may vary. It is advisable to titrate the antibody for each application and new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including this UBE2F-targeting polyclonal antibody. Our portfolio encompasses validated antibody pairs, bulk ancillary reagents, and hard-to-find targets for the neddylation and ubiquitin-proteasome research community. For customized sourcing, technical consulting, or scale-up needs, reach out to explore how we can accelerate your assay development from concept to clinic.