UBE2D1 Rabbit pAb for WB, IHC-P, ELISA - P51668

Core Product Specifications and Parameters

Biological Background: UBE2D1 Function and Localization

• UBE2D1 (Ubiquitin-conjugating enzyme E2 D1), also known as UBCH5, UBC4/5, SFT, or E2(17)KB1, is a member of the ubiquitin-conjugating enzyme family encoded by the UBE2D1 gene.
• It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to target proteins, including forming 'Lys-48'-linked polyubiquitin chains. Related references: PMID:22496338, PMID:20061386
• This enzyme mediates the selective degradation of short-lived and abnormal proteins and participates in the E6/E6-AP-dependent ubiquitination of p53/TP53.
• UBE2D1 ubiquitinates a range of substrates, including PEX5, STUB1, TRAF6, TRIM63/MURF1, HSP90AB1, and CYP3A4, and undergoes auto-ubiquitination. Related references: PMID:18042044, PMID:18359941, PMID:19103148
• It lacks inherent specificity for any particular lysine residue of ubiquitin, which allows the generation of diverse ubiquitin chain topologies. Related references: PMID:18042044
• Essential for the viral activation of IRF3, highlighting a role in innate immunity. Related references: PMID:19854139
• UBE2D1 is predominantly located in the cytoplasm and is ubiquitously expressed, with elevated levels in the liver of iron-overloaded hemochromatosis patients.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-147 of human UBE2D1) covers nearly the full-length protein, making the antibody suitable for detecting both native and denatured forms in WB and IHC-P.
• For WB, the predicted protein weight is 17 kDa; consider using appropriate molecular weight markers and optimize loading controls for small proteins.
• For IHC-P, validate fixation and antigen retrieval conditions for your specific tissue types; cytoplasmic staining is expected as positive signal.
• For ELISA, titrate the antibody concentration using the recombinant immunogen as a reference standard.
• Reported cross-reactivity with mouse and rat should be confirmed by sequence alignment and preliminary experiments in your sample system.
• Given the ubiquitous expression of UBE2D1, include negative controls (tissue/cell line with low or absent expression) or use knockdown/knockout validation to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For researchers and developers studying ubiquitin-mediated protein degradation, CamelBio can support your workflow from concept to clinic with reliable UBE2D1 antibodies and related reagents. Our one-stop sourcing streamlines procurement and accelerates assay development.