Tyro3 Rabbit Polyclonal Antibody for WB, ELISA - Q06418

Core Product Specifications and Parameters

Biological Background: Tyro3 Function and Localization

• Tyro3 is a tyrosine-protein kinase receptor also known as BYK, DTK, RSE, SKY, or TIF, and belongs to the TAM receptor family.
• It functions as a receptor tyrosine kinase that transduces signals from the extracellular matrix upon binding to ligands such as GAS6 or TULP1, regulating cell survival, migration, and differentiation.
• Ligand binding induces receptor dimerization and autophosphorylation, leading to activation of downstream pathways including PI3K-AKT signaling and nuclear translocation of NF-kappa-B, promoting cell survival.
• Tyro3 plays a protective role in neurons against excitotoxic injury and is involved in platelet aggregation and cytoskeleton reorganization.
• It inhibits Toll-like receptor (TLR)-mediated innate immune responses by activating STAT1, which induces suppressors of cytokine signaling SOCS1 and SOCS3.
• Acts as a host cell receptor for Lassa virus, lymphocytic choriomeningitis virus, and Ebolavirus, likely via GAS6-mediated recognition of phosphatidylserine on viral envelopes.
• Tyro3 is primarily localized at the cell membrane and is abundant in the brain, with lower expression in other tissues.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 290-430, a region within the extracellular domain, potentially recognizing multiple isoforms or cleavage products; verify the detected band size in your experimental system.
• For Western blot (WB), use reducing conditions and whole-cell lysates from tissues or cell lines endogenously expressing Tyro3 (e.g., brain homogenates) to confirm specific detection. Cross-reactivity with mouse and rat makes this antibody suitable for rodent models.
• In ELISA applications, ensure proper titration and consider using the antibody as a capture or detection reagent after validating its performance in your assay format.
• Because Tyro3 has roles in both neurobiology and viral entry, include appropriate positive controls (e.g., transfected cells, known positive tissue lysates) and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For Tyro3-related neurobiology and infectious disease research, we offer validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to accelerate your assay development. Where standard catalog products are not available, our team can source rare-target raw materials to meet your specific project needs.