TSPAN15 Rabbit pAb for WB and ELISA - O95858

Core Product Specifications and Parameters

Biological Background: TSPAN15 Function and Localization

• Tetraspanin-15 (Tspan-15), also known as NET-7 or TM4SF15, is a member of the tetraspanin family and belongs to the TspanC8 subgroup, which consists of six members that directly interact with the transmembrane metalloprotease ADAM10. Related references: PMID:26686862, PMID:30463011, PMID:31792032
• Tspan-15 is required for ADAM10 exit from the endoplasmic reticulum, enzymatic maturation, trafficking to the cell surface, and the determination of substrate specificity. Different TspanC8/ADAM10 complexes exhibit distinct substrate preferences. Related references: PMID:26686862, PMID:30463011, PMID:31792032
• TSPAN15 promotes ADAM10-mediated cleavage of CDH2 (N-cadherin), influencing cell adhesion and migration. Related references: PMID:34739841
• The protein negatively regulates ligand-induced Notch signaling activity, likely by modulating ADAM10 activity. Related references: PMID:26686862, PMID:31792032
• TSPAN15 localizes primarily to the cell membrane and late endosome membrane.
• The protein contains several transmembrane helices and disulfide bonds, and it is classified as a glycoprotein. Its mature molecular weight is approximately 33 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110-220 of human TSPAN15, which lies within the large extracellular loop (EC2) that mediates crucial protein–protein interactions. This design may favor recognition of native conformations in whole‑cell contexts.
• For WB, consider the predicted molecular weight of ~33 kDa and validate detection with appropriate positive controls (e.g., cell lines expressing ADAM10/TspanC8 components). Given the polyclonal nature, background bands might appear; pre‑absorption or optimized blocking can be evaluated.
• In ELISA, the recombinant immunogen or a synthetic peptide covering the target region can serve as positive coating antigens. Confirm reactivity against native protein in lysates or purified membranes from human, mouse, or rat samples.
• Cross‑reactivity with mouse and rat is anticipated due to high sequence homology in the immunogen region; nevertheless, verify specificity using knockout or knockdown models.
• The antibody’s ability to immunoprecipitate the ADAM10–TSPAN15 complex under native conditions has not been tested; buffer conditions should be optimized if this application is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing validated antibody pairs, bulk ancillary reagents, and rare‑target raw‑material sourcing across the IVD development pipeline. For tetraspanin biology and ADAM10/Notch pathway research, we offer a curated portfolio of antibodies and complementary critical raw materials. Contact us to streamline your procurement and accelerate development from concept to clinical application.