TSFM Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P43897

Core Product Specifications and Parameters

Biological Background: TSFM Function and Localization

• TSFM encodes mitochondrial elongation factor Ts (EF-Tsmt), a key component of the mitochondrial translation machinery.
• It associates with the EF-Tu.GDP complex and catalyzes the exchange of GDP for GTP, facilitating the regeneration of active EF-Tu.GTP required for aminoacyl-tRNA delivery to the ribosome. Related references: PMID:27677415
• The protein localizes to the mitochondrial matrix, where it participates in protein synthesis within the organelle.
• TSFM is ubiquitously expressed, with the highest levels in skeletal muscle, liver, and kidney, reflecting the high metabolic demand of these tissues.
• Alternative splicing produces multiple isoforms, and the protein is subject to phosphorylation (Phosphoprotein keyword).
• Mutations in TSFM are associated with combined oxidative phosphorylation deficiency and cardiomyopathy, highlighting its critical role in mitochondrial function.

Experimental Guidance and Technical Tips

• The immunogen, located within the N-terminal 1-100 amino acids, may be accessible in both native and denatured conformations, supporting applications such as WB and IP.
• For Western blotting, the predicted molecular weight is ~35 kDa; consider using mitochondrial-enriched lysates from human, mouse, or rat tissues to enhance signal.
• For immunofluorescence/ICC, anticipate mitochondrial localization; co-staining with a mitochondrial marker is recommended to confirm specificity.
• For immunoprecipitation, validate the antibody in the appropriate cell or tissue lysate system, as binding efficiency can vary with sample preparation.
• ELISA performance may be optimized by using peptide-coated plates or recombinant protein; consider pairing with a detection antibody for sandwich assays.

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CamelBio provides a comprehensive sourcing platform for IVD raw materials, including validated monoclonal antibodies like this anti-TSFM clone for mitochondrial research. Our portfolio covers antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support diagnostic development from concept to clinic.