TRUB1 Rabbit pAb - Q8WWH5

Core Product Specifications and Parameters

Biological Background: TRUB1 Function and Localization

• TRUB1 (TruB pseudouridine synthase homolog 1, also referred to as PUS4) is a pseudouridylate synthase that catalyzes pseudouridylation of both mRNAs and tRNAs. Related references: PMID:28073919, PMID:31477916, PMID:32926445

• It mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3' harboring a stem-loop structure, serving as the major pseudouridine synthase on mRNAs. Related references: PMID:28073919, PMID:31477916

• TRUB1 catalyzes pseudouridylation of specific tRNAs, including synthesis of pseudouridine(55) from uracil-55 in the psi GC loop of a subset of tRNAs. Related references: PMID:32926445, PMID:33023933

• Independently of its RNA pseudouridylate synthase activity, TRUB1 promotes processing of pri-let-7 microRNAs by binding to the stem-loop structure, preventing LIN28-binding, and enhancing DGCR8 interaction. Related references: PMID:32926445

• It localizes to both the nucleus and the cytoplasm (cytosol).

• TRUB1 is highly expressed in heart, skeletal muscle, and liver, with lower levels in lung, small intestine, kidney, and spleen.

• Keywords associated with TRUB1 include isomerase, mRNA processing, tRNA processing, acetylation, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 100-280) of human TRUB1, which shares high homology with mouse and rat orthologs, supporting the observed cross-reactivity; validate sequence alignment in your target species.
• For Western Blot, a band around 37 kDa is expected; consider using tissue lysates with high TRUB1 expression (e.g., heart, skeletal muscle, liver) for initial validation.
• In ELISA, consider immobilizing recombinant TRUB1 protein or cell/tissue extracts; perform checkerboard titrations to optimize coating and antibody concentrations.
• Since TRUB1 is a pseudouridylase, confirm band specificity using appropriate controls (e.g., knockdown/knockout lysates if available) to distinguish from non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating mRNA/tRNA modifications or miRNA processing, this TRUB1 polyclonal antibody is a valuable tool that can be integrated into your assay development workflow. CamelBio's supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, streamlining your path from discovery to diagnostic application.