TRMT112 Rabbit Polyclonal Antibody for WB, ELISA - Q9UI30

Core Product Specifications and Parameters

Biological Background: TRMT112 Function and Localization

• TRMT112, also known as tRNA methyltransferase 112 homolog (TRM112; HSPC152; HSPC170; hTrm112; TRMT11-2), is a multifunctional methyltransferase activator partner that forms heterodimers with various methyltransferases to modify rRNA, tRNA, and proteins. Related references: PMID:18539146, PMID:20308323, PMID:25851604
• The TRMT112-BUD23 complex methylates guanine at the N(7) position within 18S rRNA, a modification essential for ribosome biogenesis and function. Related references: PMID:25851604
• Together with N6AMT1/HEMK2, TRMT112 catalyzes N5-methylation of eukaryotic translation termination factor 1 (ETF1) on glutamine-185, using S-adenosyl-L-methionine as the methyl donor. Related references: PMID:18539146, PMID:31061526, PMID:31636962
• This TRMT112-N6AMT1 heterodimer also monomethylates lysine-12 of histone H4 (H4K12me1), linking TRMT112 to epigenetic regulation and chromatin dynamics. Related references: PMID:31061526
• With ALKBH8, TRMT112 methylates 5-carboxymethyluridine to 5-methylcarboxymethyluridine at the tRNA wobble position, crucial for decoding efficiency. Related references: PMID:20308323
• In partnership with THUMPD3, TRMT112 facilitates N(2)-methylguanosine formation at position 6 of multiple tRNAs and position 7 of tRNA(Trp). Related references: PMID:34669960, PMID:37283053
• TRMT112 also teams with METTL5 to methylate adenosine 1832 of 18S rRNA, contributing to small ribosomal subunit maturation. Related references: PMID:31328227, PMID:33428944, PMID:35033535
• Beyond its methyltransferase activation roles, TRMT112 participates in pre-rRNA processing steps essential for small-subunit rRNA production. Related references: PMID:25851604
• Subcellularly, TRMT112 is found in the nucleoplasm, perinuclear cytoplasm, and nucleus, aligning with its dual functions in nuclear RNA modification and cytoplasmic translation regulation.
• TRMT112 is a phosphoprotein subject to alternative splicing, as identified through proteomics and structural analyses.

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to the predicted molecular weight of approximately 14 kDa is expected. Optimize blocking and antibody dilution in the relevant sample system.
• This polyclonal antibody is suitable for ELISA applications; appropriate secondary detection and optimization of coating antigen conditions are recommended.
• Cross-reactivity is reported for human, mouse, and rat; due to sequence homology, users should verify specificity in other species or isoforms as needed.
• Given the immunogen design (full-length recombinant protein), the antibody may recognize multiple epitopes, potentially offering robust detection across different sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials for epigenetic and rRNA processing studies, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. In addition to this TRMT112 polyclonal antibody, we offer sourcing support for rare-target raw materials and technical consulting to accelerate your assay development. Our integrated supply chain bridges the gap from concept to clinic, ensuring consistent quality and scalability.