TrkB Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q16620

Core Product Specifications and Parameters

Biological Background: TrkB Function and Localization

• TrkB (NTRK2) is a receptor tyrosine kinase that mediates neurotrophin signaling, acting as a receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NTF4), and with lower affinity for neurotrophin-3 (NTF3) (PubMed:15494731, PubMed:7574684). Related references: PMID:15494731 PMID:7574684

• Upon ligand binding, TrkB homodimerizes and undergoes autophosphorylation, recruiting and activating downstream effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1, which propagate signaling through the GRB2-Ras-MAPK, PI3K-AKT1, and PLCG1-PKC pathways (PubMed:15494731). Related references: PMID:15494731

• TrkB signaling regulates neuron survival, proliferation, migration, differentiation, neurite outgrowth, and synaptic plasticity, playing critical roles in central and peripheral nervous system development and function.

• TrkB is essential for learning and memory, modulating both short-term synaptic function and long-term potentiation (LTP), with PLCG1-mediated signaling contributing to NF-kappa-B activation and anti-apoptotic gene transcription.

• Subcellular localization includes the cell membrane, endosome membranes (early endosome), axonal and dendritic projections, postsynaptic density, and perinuclear cytoplasm.

• Multiple isoforms exist: full-length TrkB is widely expressed in CNS and PNS; TrkB-T1 is detected in brain, pancreas, kidney, and heart; TrkB-T-Shc is predominantly brain-specific.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 32-430), suggesting the antibody may recognize the extracellular region and could be suitable for detecting both full-length and secreted forms, but further verification is recommended.
• For Western blotting, the predicted molecular weight is ~92 kDa; however, glycosylation and isoform variations may cause bands at different sizes. Validate with appropriate positive controls.
• For IHC-P, optimize antigen retrieval conditions and consider testing both paraffin-embedded and frozen sections to confirm specificity in tissue.
• Since cross-reactivity covers human, mouse, and rat, this antibody is a candidate for translational neuroscience studies; however, validate in the specific sample system before quantitative analysis.
• For ELISA, use as capture or detection antibody in matched-pair screening with appropriate standard proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this TrkB rabbit monoclonal antibody along with complementary IVD raw materials to support neuroscience research and diagnostic development. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. From single antibodies to full kitting, CamelBio serves as your one-stop sourcing bridge from concept to clinic.