TrkA Rabbit Monoclonal Antibody for WB, ELISA - P04629

Core Product Specifications and Parameters

Biological Background: TrkA Function and Localization

• TrkA, encoded by the NTRK1 gene, is the high affinity nerve growth factor (NGF) receptor tyrosine kinase. It is also known as neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, tropomyosin-related kinase A, and p140-TrkA (UniProt: P04629).
• Upon NGF binding, TrkA homodimerizes and autophosphorylates, triggering downstream signaling cascades, including the Ras-MAPK pathway (cell differentiation and survival), PI3K-AKT pathway (survival), and PLCG1-mediated NF-kappa-B activation (PubMed:1281417, PubMed:15488758, PubMed:17196528).
  Related references:
  PMID:1281417 PMID:15488758 PMID:17196528
• Three major isoforms exist: TrkA-I (widely expressed), TrkA-II (predominantly neuronal), and TrkA-III (pluripotent neural stem/progenitor cells). Isoform TrkA-III lacks the extracellular NGF-binding domain, promoting angiogenesis and oncogenic activity.
• Subcellularly, TrkA localizes to the cell membrane and is internalized into early, late, and recycling endosomes upon activation.
• TrkA is critical for nervous system development, and chromosomal rearrangements involving NTRK1 are implicated in certain cancers. It is classified as a proto-oncogene and undergoes alternative splicing, glycosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular region (amino acids 697–796) of human TrkA, which is highly conserved in mouse and rat, supporting observed cross-reactivity.
• For Western blotting, the predicted molecular weight is approximately 87 kDa. Due to glycosylation, the observed band may appear slightly higher. Reducing conditions and appropriate blocking are recommended.
• In ELISA applications, consider pairing with a suitable capture antibody and optimizing coating conditions. Validate in relevant biological matrices.
• As a rabbit monoclonal antibody, it offers high specificity and batch-to-batch consistency for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, clinical laboratories, and research institutes with integrated access to IVD raw materials, technical services, and consulting. This high-affinity TrkA recombinant rabbit monoclonal antibody supports neuroscience and oncology research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.