TRIOBP Polyclonal Antibody for WB, IHC-P, ELISA - Q9H2D6

Core Product Specifications and Parameters

Biological Background: TRIOBP Function and Localization

• TRIOBP (Tara, TAP68) is an actin-binding protein that directly stabilizes filamentous F-actin and prevents its depolymerization, regulating cell spreading and contraction. Related references: PMID:18194665, PMID:28438837
• It also functions as a linker protein, recruiting factors required for F-actin formation and turnover. Related references: PMID:18194665
• TRIOBP is essential for correct mitotic progression, ensuring proper chromosome segregation. Related references: PMID:22820163, PMID:24692559
• Plays a pivotal role in the formation of stereocilia rootlets in inner ear hair cells.
• Localizes to multiple subcellular compartments: nucleus, cytoplasm, centrosome, midbody, and telomeres.
• Widely expressed with high levels in heart and placenta; also detected in fetal brain, retina, and cochlea.
• Undergoes alternative promoter usage and splicing, producing tissue-specific isoforms.
• Associated with non-syndromic deafness (DFNB28) and is subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180-310 of human TRIOBP (NP_008963.3). Consider that the polyclonal antibody may recognize multiple isoforms or species orthologs.
• For Western blotting (WB), a predicted band around 261 kDa is expected. Use appropriate high-molecular-weight markers and gradient gels to resolve this large protein. Validate detection in lysates from heart or placenta, which express TRIOBP highly.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; optimize using positive control tissues such as retina or cochlea where expression is reported.
• For ELISA, pair with an appropriate coating antigen and validate against recombinant TRIOBP fragments to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a seamless procurement experience for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents from concept to clinic. This anti-TRIOBP polyclonal antibody supports research into actin dynamics, mitosis, and hearing loss. Beyond single antibodies, we supply validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and specialized sourcing for rare targets like TRIOBP isoforms—ensuring your project’s success with a comprehensive supply chain.