TriMethyl-Histone H4-K20 Rabbit Monoclonal Antibody (P62805) for WB, IHC-P, IF/ICC

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, the fundamental repeating unit of chromatin. It wraps and compacts DNA, thereby limiting accessibility to the cellular machineries that require DNA as a template. Consequently, H4 plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt:P62805

The N-terminal tail of histone H4, including lysine 20 (K20), is subject to a vast array of post-translational modifications—acetylation, methylation, phosphorylation, and more—that constitute the "histone code." These modifications dynamically regulate chromatin structure and gene expression. Related references: PMID:12086618, PMID:17967882

Specifically, trimethylation at lysine 20 (H4K20me3) is a well-characterized epigenetic mark primarily associated with constitutive heterochromatin, contributing to gene silencing and maintaining genome stability. This modification is catalyzed by methyltransferases such as SUV420H1 and SUV420H2. Related references: PMID:12086618, PMID:15964846, PMID:17967882, PMID:32209475

Subcellularly, histone H4 is predominantly localized to the nucleus and chromosomes. During spermatogenesis, acetylated H4 exhibits enhanced nuclear retention in step 11 spermatids. Related references: UniProt:P62805

Experimental Guidance and Technical Tips

• Because this antibody recognizes a methylation-specific epitope, ensure blocking and washing buffers maintain methyl group stability. Non-fat dry milk or BSA may be used, but avoid harsh stripping conditions that could alter the modification.
• For Western blot, consider using acid-extracted histones or nuclear-enriched lysates to boost signal of histone modifications, and run samples on high-percentage (15% or gradient) SDS-PAGE for optimal resolution of the 11 kDa H4 protein.
• In IHC-P, standard antigen retrieval methods (e.g., citrate buffer pH 6.0) are typically compatible; however, validate in tissue sections known to express H4K20me3 marks, such as heterochromatic regions.
• For ELISA or dot blot applications, include non-methylated and mono-/di-methylated peptide controls to confirm trimethyl specificity.
• This recombinant rabbit monoclonal format offers high reproducibility for long-term studies; validate dilution factors in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with seamless access to validated antibody products like this TriMethyl-Histone H4-K20 Rabbit mAb. Our extensive portfolio includes optimized monoclonal/polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents tailored for epigenetic and chromatin biology applications. For targets with limited commercial availability, CamelBio specializes in rare raw-material sourcing, bridging global innovation from concept to clinic with reliable supply solutions.