TriMethyl-Histone H3 (K9) Rabbit Polyclonal Antibody - Q16695/P68431 | WB, ChIP, IHC, ELISA

Core Product Specifications and Parameters

Biological Background: TriMethyl-Histone H3-K9 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to limit DNA accessibility. This compaction imposes a central role for histones in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is dynamically regulated through a complex set of post-translational modifications (the "histone code") and nucleosome remodeling. Related references: UniProt Q16695 / P68431

Human histone H3 exists as multiple variants, including the canonical replication-dependent H3.1 (encoded by several genes such as H3C1, H3C2, etc.; UniProt P68431) and the testis-specific H3.1t (gene H3-4; UniProt Q16695). Both variants share a highly conserved N-terminal tail that is subject to extensive post-translational modifications. Related references: UniProt Q16695 / P68431

The trimethylation of lysine 9 (H3K9me3, corresponding to position 10 in the full-length UniProt sequence) is a hallmark modification frequently associated with constitutive heterochromatin formation, transcriptional silencing, and the recruitment of HP1 (heterochromatin protein 1). This modification has been documented in UniProt with extensive experimental evidence. Related references: PMID:11242053, PMID:16185088, PMID:16267050, PMID:16457588, PMID:17194708

Both H3.1 and H3.1t localize to the nucleus and associate with chromosomes. While H3.1 is broadly expressed, H3.1t expression is restricted to testicular cells. Related references: UniProt Q16695 / P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide containing trimethylated K9; for Western blot, consider using whole‑cell lysates or histone‑enriched extracts and validate the expected band at ~15 kDa.
• For chromatin immunoprecipitation (ChIP), design qPCR primers for known H3K9me3‑enriched loci (e.g., pericentromeric repeats) as positive controls; include an unmodified peptide competition to verify antibody specificity.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), optimize fixation, permeabilization, and antigen retrieval conditions for your sample; positive and negative control tissues (e.g., testis vs. non‑testis) are recommended.
• The antibody is predicted to cross‑react with a wide range of species beyond human, mouse, and rat; therefore, validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This trimethyl‑H3K9 antibody addresses the growing need in epigenetics and chromatin research for reliable PTM‑specific detection tools. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare‑target raw materials—ensuring a streamlined supply chain for your diagnostic development.