TriMethyl-Histone H3-K79 Rabbit Polyclonal Antibody (Q16695 / P68431) for WB, ChIP, Epigenetics

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. It thereby plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt Q16695, UniProt P68431

DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H3 is subject to numerous modifications including methylation, acetylation, phosphorylation, and more. Related references: UniProt Q16695, UniProt P68431

Histone H3 localizes to the nucleus and chromosome. The H3.1t variant (Q16695) is specifically expressed in testicular cells, while canonical H3.1 (P68431) is ubiquitous. Related references: UniProt Q16695, UniProt P68431

Methylation at lysine 79 (K79) is an evolutionarily conserved histone mark associated with active transcription and DNA damage response. This antibody specifically recognizes the trimethylated form.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic trimethylated peptide around K79 of human histone H3. Consider using this antibody to detect endogenous H3K79me3 levels in various cell lines and tissues.
• For Western blotting (WB), a band at ~15 kDa is expected. Validate with appropriate positive and negative controls (e.g., cells treated with methyltransferase inhibitors).
• For chromatin immunoprecipitation (ChIP), this antibody may enrich genomic regions with active transcription marks; optimize crosslinking and sonication conditions.
• In ELISA, the antibody can be used to detect trimethylated peptides; ensure coating and blocking steps are optimized.
• Cross-reactivity is predicted for a wide range of species due to sequence conservation of the K79 region; confirm in your target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For epigenetic research and histone modification analysis, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development. Our global supply chain can source rare-target raw materials like this TriMethyl-Histone H3-K79 antibody, ensuring consistent quality from R&D to commercial production.