TriMethyl-Histone H3-K56 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, IF/ICC, ELISA, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H3 (H3.1/H3.1t) Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to limit DNA accessibility. It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• DNA accessibility is governed by a complex set of post-translational modifications (PTMs) on histones, collectively referred to as the histone code. These PTMs are read by effector proteins that mediate chromatin state changes and gene expression outcomes.
• Trimethylation of lysine 56 (K56me3) is a specific PTM located in the globular domain of H3. This modification can influence histone-DNA interactions within the nucleosome and is implicated in DNA damage response and chromatin dynamics during replication.
• The canonical histone H3.1 is ubiquitously expressed, while the variant H3.1t (encoded by H3-4/HIST3H3) shows testis-specific expression.
• Both isoforms localize to the nucleus and associate with chromosomes, consistent with their structural role in chromatin organization.

Related references:

• H3K56me3 identification by mass spectrometry PMID:17194708
• Role of EHMT2 (G9a) in K56 methylation PMID:22387026
• Nucleosome core particle composition and histone modifications (review) UniProt entry

Experimental Guidance and Technical Tips

• Consider using a peptide competition assay with both trimethylated and unmodified K56 peptides to confirm the specificity of the antibody for the K56me3 mark, particularly when working with complex biological samples.
• For Western blotting, validate the observed band at the expected 15 kDa molecular weight using nuclear extracts from species of interest, and include appropriate loading controls and molecular weight markers.
• In ChIP-seq experiments, include a control reaction using an unmodified peptide or an antibody-blocked sample to rule out nonspecific enrichment. Cross-reactivity across a wide range of species is predicted due to high sequence conservation of histone H3, but experimental validation in the target species is advised.
• For IF/ICC applications, consider co-staining with markers of chromatin or specific nuclear substructures to confirm spatial localization of the signal.

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CamelBio connects diagnostic manufacturers, research laboratories, and institutes with a reliable, one-stop platform for IVD raw-material sourcing. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target raw-material sourcing, we support your entire development workflow—from concept to clinic. The TriMethyl-Histone H3-K56 Rabbit pAb exemplifies our specialized antibody portfolio for epigenetic research and diagnostic assay development, addressing the needs of chromatin biology and histone modification analysis.