TriMethyl-Histone H3-K4 Rabbit mAb - Q16695/P68431 - WB, IHC-P, IF, ChIP Antibody

Core Product Specifications and Parameters

Biological Background: TriMethyl-Histone H3-K4 Function and Localization

Histone H3 is a core component of the nucleosome. Nucleosomes wrap and compact DNA into chromatin, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications, known as the histone code, and nucleosome remodeling.
Related references: UniProt Q16695, UniProt P68431

Trimethylation of histone H3 at lysine 4 (H3K4me3) is an epigenetic mark associated with active gene promoters and transcriptional initiation. This modification is catalyzed by histone methyltransferases and is recognized by various chromatin-binding proteins.
Related references: PMID:16267050, PMID:16457588, PMID:17194708

Histone H3 is predominantly localized to the nucleus and associates with chromosomes during cell division. The H3.1t variant (Q16695) is expressed in testicular cells, while canonical H3.1 (P68431) is expressed ubiquitously.
Related references: UniProt Q16695, UniProt P68431

Multiple post-translational modifications on histone H3, including methylation, acetylation, phosphorylation, and citrullination, dynamically regulate chromatin structure and function. The H3K4 site specifically undergoes mono-, di-, and trimethylation, with trimethylation being a hallmark of active transcription.
Related references: PMID:16267050, PMID:16457588, PMID:17194708

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to the trimethylated K4 region of human histone H3. This ensures specific recognition of the H3K4me3 mark.
• For Western blotting (WB), a band at approximately 15 kDa, consistent with the molecular weight of histone H3, may be detected. This antibody is also recommended for IHC-P, IF/ICC, ELISA, and ChIP applications. Researchers should optimize dilution and assay conditions in their experimental system.
• The antibody is predicted to cross-react with mouse and rat due to high sequence conservation of the H3K4 region; preliminary validation in the relevant species is advised.
• When performing ChIP, the antibody has been reported to enrich DNA sequences associated with H3K4me3; consider it for studying promoter regions and active genes.
• As with any post-translational modification-specific antibody, confirm specificity using appropriate controls (e.g., peptide competition, genetic knockout/knockdown, or treatment with histone-modifying enzyme inhibitors).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes a wide range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This TriMethyl-Histone H3-K4 antibody supports epigenetic research and chromatin-based assay development. If you require customized sourcing solutions or have specific assay needs, our team is ready to assist in bridging your concept to clinic.