TriMethyl-Histone H3-K4 (Q16695 / P68431) Rabbit Monoclonal Antibody for Epigenetic Research & ChIP, WB, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to restrict accessibility for transcriptional, repair, and replication machinery. It plays a central role in maintaining chromosomal stability and regulating gene expression through a complex array of post‑translational modifications (the "histone code"). Related references: PMID:17194708
• The canonical H3.1 protein (encoded by multiple HIST1H3 genes, SWISS P68431) is expressed in replication‑dependent manner across most cell types, while the testis‑specific variant H3.1t (HIST3H3, SWISS Q16695) is predominantly expressed in testicular cells. Both localize to the nucleus and associate with chromosomes during cell division. Related references: Gene/Protein specific; see UniProt Q16695/P68431
• Trimethylation at lysine 4 (H3K4me3) is a pivotal epigenetic mark associated with active promoters, open chromatin conformation, and transcriptional initiation. It is deposited by COMPASS‑family methyltransferases and recognized by reader proteins that facilitate gene activation. Related references: PMID:16185088
• H3K4me3 coexists and crosstalks with other histone modifications (e.g., acetylation, phosphorylation, methylation at other residues) to fine‑tune chromatin states. Dysregulation of this mark has been implicated in cancer, developmental disorders, and immune dysfunction, making it a key biomarker in epigenetic research and IVD development.

Experimental Guidance and Technical Tips

• The immunogen (a synthetic trimethylated peptide around K4) was designed to confer high specificity for the H3K4me3 modification. To confirm selectivity, consider peptide competition assays or treatment with histone demethylases (e.g., LSD1) that remove methyl groups.
• For Western blot (WB), histone extraction protocols that enrich for basic nuclear proteins are recommended. The expected band is ~15 kDa; verify band identity using H3K4me3‑positive controls.
• In chromatin immunoprecipitation (ChIP), validate enrichment at known H3K4me3 target genes (e.g., GAPDH promoter) by qPCR. Include appropriate IgG controls to assess background.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), antigen retrieval conditions (pH 6 citrate or pH 9 EDTA) may influence staining. Test a range of dilutions on positive and negative tissues.
• Due to high sequence conservation across species, cross‑reactivity with human, mouse, rat, and other vertebrates is expected. Validate detection in your specific sample system before quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering a streamlined pathway from concept to clinic. Our portfolio spans validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized rare target reagents to power your diagnostic and research workflows. This TriMethyl-Histone H3-K4 Rabbit mAb exemplifies our methylated antibody collection, designed to support epigenetic biomarker research and ChIP‑based assay development with the reliability and performance you need.