TriMethyl-Histone H3-K36 Rabbit Polyclonal Antibody for Epigenetic Research (Q16695/P68431)

Core Product Specifications and Parameters

Biological Background: Histone H3.1t and H3.1 Function and Localization

Histone H3.1 and its testis-specific variant H3.1t are core components of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, regulating access for transcription, replication, and repair machinery. The dynamic post-translational modification (PTM) landscape of histones, often referred to as the "histone code," governs genomic functions and ultimately influences cellular identity and stability.

Related references: PMID:17194708, PMID:22387026

Both H3.1 and H3.1t localize to the nucleus and are integral to chromosome structure. While H3.1 is ubiquitously expressed across somatic cells, H3.1t is selectively expressed in the testis, suggesting a specialized role in chromatin remodeling during spermatogenesis.

Related references: PMID:16185088, PMID:15983376

Trimethylation of lysine 36 (H3K36me3) is a prominent histone mark associated with actively transcribed gene bodies, DNA repair, and recombination. This modification is deposited by SETD2 methyltransferase and recognized by reader proteins that mediate downstream effects on splicing and chromatin compaction.

Related references: PMID:16267050, PMID:16627869

Dysregulation of H3K36 methylation has been linked to several cancers and developmental disorders, making it a critical target for epigenetic research and diagnostic applications.

Experimental Guidance and Technical Tips

• For Western blot (WB), the antibody detects a band at approximately 15 kDa. Consider using whole-cell lysates or histone-enriched extracts from human, mouse, or rat cells.
• When performing ChIP or ChIP-seq, titrate antibody amount and validate enrichment of target loci (e.g., actively transcribed genes) alongside positive and negative control regions.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), fixation and antigen retrieval conditions may need optimization; test permeability and blocking protocols for your specific sample.
• The broad species cross-reactivity (human, mouse, rat, and wide-range predicted) suggests this antibody may recognize conserved epitopes, but always verify specificity in non-validated species.
• As a methylation-specific reagent, cross-reactivity with unmodified or differently modified H3 should be ruled out using appropriate peptide competition or knockout models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a seamless one-stop supply channel for high-quality IVD raw materials. Whether you need validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, or rare-target sourcing, our integrated platform accelerates your project from concept to clinic. This TriMethyl-Histone H3-K36 antibody exemplifies our commitment to providing precise, application-tested tools for chromatin and epigenetic investigations. Partner with CamelBio to streamline your R&D and secure all critical reagents under a single reliable source.