TriMethyl-Histone H3-K36 (K36me3) Rabbit mAb, Q16695/P68431 – WB, ChIP-seq, IHC-P

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping DNA into chromatin and playing a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Related references: UniProtKB Q16695, UniProtKB P68431
• The protein localizes to the nucleus and chromosomes. Related references: UniProtKB Q16695, UniProtKB P68431
• Histone H3.1t (encoded by H3-4/HIST3H3, accession Q16695) is testis-specific, while canonical H3.1 (P68431) is expressed ubiquitously.
• The N-terminal tail of histone H3 is subject to extensive post-translational modifications (PTMs), including methylation, acetylation, phosphorylation, and others, which constitute the "histone code" that regulates chromatin dynamics. Related references: PMID:16267050, PMID:17194708
• Methylation of lysine residues such as K36 is associated with active transcription elongation and is often enriched in gene bodies. Trimethylation at K36 (H3K36me3) serves as a binding platform for various reader proteins involved in transcription and splicing. Related references: PMID:16267050
• This antibody, raised against a synthetic trimethylated peptide around K36, specifically recognizes the K36me3 modification, enabling detailed study of this epigenetic mark.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic trimethylated peptide corresponding to residues around K36 of human Histone H3. When performing assays, consider including a non-methylated peptide or a different methylation state peptide as a negative control to confirm specificity.
• For Western blot (WB), acid-extracted histones or whole cell lysates are suitable sample types. The expected band size is ~15 kDa. Optimize loading and antibody dilution for your specific system.
• This antibody is suitable for ChIP and ChIP-seq; follow standard chromatin preparation and fragmentation protocols, and consider using spike-in controls for normalization.
• Cross-reactivity is predicted for a wide range of species beyond human, mouse, and rat due to high sequence conservation. However, validation in the intended species is recommended.
• For immunohistochemistry (IHC-P), antigen retrieval and detection conditions may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research institutions with high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies like this TriMethyl-Histone H3-K36 rabbit mAb. For your epigenetic assay development needs, CamelBio offers one-stop access to optimized antibody pairs, bulk ancillary reagents, and custom raw-material sourcing, streamlining your journey from concept to clinic.