TriMethyl-Histone H3-K27 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, ChIP, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Related references: UniProt P68431

Histone H3 localizes to the nucleus and is associated with chromosomes throughout the cell cycle. Related references: UniProt P68431

Multiple isoforms of histone H3 are encoded by a family of genes. The canonical H3.1 (encoded by several H3C genes, SWISS P68431) is widely expressed, while H3.1t (Q16695, also known as H3t or H3/g) is specifically expressed in testicular cells. Related references: UniProt Q16695

Trimethylation at lysine 27 (K27) of histone H3 is a critical epigenetic mark. In the canonical H3.1 and H3.1t sequences, this corresponds to position 28. This modification is associated with transcriptional repression and is dynamically regulated by methyltransferases and demethylases. Related references: PMID:16185088, PMID:16267050, PMID:16627869

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human histone H3. Given the high conservation of histone H3 across species, this antibody is predicted to detect a wide range of mammalian species (human, mouse, rat, and others).
• This antibody is designed to recognize trimethylated K27 specifically. When performing applications such as Western blotting or ChIP, consider including appropriate controls (e.g., non-methylated peptide competition or lysate from cells with altered methylation status) to confirm specificity.
• For ChIP assays, optimize chromatin shearing and antibody concentration to ensure specific enrichment of K27-trimethylated chromatin regions.
• In immunofluorescence or immunohistochemistry, fixation and permeabilization conditions may affect epitope accessibility. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This TriMethyl-Histone H3-K27 Rabbit pAb (Q16695/P68431) exemplifies our commitment to supplying high-quality antibodies for epigenetic research. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD reagent development and discovery workflows.