TriMethyl-Histone H3-K27 Rabbit Monoclonal Antibody (Q16695/P68431) for ChIP, CUT&Tag, WB

Core Product Specifications and Parameters

Biological Background: TriMethyl-Histone H3 (K27) Function and Localization

Histone H3 is a core histone protein that, together with H2A, H2B, and H4, forms the nucleosome, the fundamental repeating unit of chromatin. As a core component of the nucleosome, histone H3 wraps DNA, condensing it into chromatin and regulating access to the underlying genetic information. This structural role is critical for maintaining chromosome stability and controlling key nuclear processes including transcription, DNA replication, and DNA repair.

Histone H3 is subject to a vast array of post-translational modifications (PTMs) at its flexible N-terminal tail, including methylation, acetylation, phosphorylation, and citrullination. These modifications, often referred to as the “histone code,” dynamically regulate chromatin structure and function. The specific trimethylation of lysine 27 (H3K27me3) is a well-characterized repressive mark that is deposited by the polycomb repressive complex 2 (PRC2) and is associated with gene silencing, X-chromosome inactivation, and developmental regulation.

The H3.1 variant (UniProt P68431) is widely expressed and constitutes the bulk of histone H3 in somatic cells, whereas H3.1t (H3-4, UniProt Q16695) is primarily expressed in testicular cells. Both variants localize to the nucleus and chromosome, where they participate in nucleosome assembly and chromatin organization.

Related references: UniProt Q16695, UniProt P68431

Experimental Guidance and Technical Tips

• Immunogen information: The antibody was raised against a synthetic trimethylated peptide spanning lysine 27 (K27) of human Histone H3. This design ensures specific recognition of the H3K27me3 modification. For applications requiring discrimination between different methylation states, validate specificity using peptide competition assays.
• Western Blot (WB): The predicted molecular weight of histone H3 is approximately 15 kDa. Use appropriate gel percentages (e.g., 15% SDS-PAGE) for optimal separation. Include positive and negative controls (e.g., cells treated with EZH2 inhibitors to reduce H3K27me3) to confirm band specificity.
• ChIP and CUT&Tag: This antibody is suitable for chromatin immunoprecipitation and CUT&Tag assays to map H3K27me3 distribution genome-wide or at specific loci. Optimize antibody concentration for each chromatin input amount and include appropriate IgG controls.
• ELISA: Use the immunizing peptide as a positive control to generate standard curves. Consider that the antibody may cross-react with peptides containing similar trimethylated motifs; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we understand the critical role of high-quality raw materials in diagnostic and research applications. This TriMethyl-Histone H3-K27 Rabbit mAb exemplifies our commitment to providing validated antibodies for epigenetic studies. Whether you need bulk ancillary reagents, optimized antibody pairs for IVD development, or assistance sourcing rare targets, CamelBio offers comprehensive supply solutions to accelerate your project from concept to clinic.