TriMethyl-Histone H3-K27 Mouse Monoclonal Antibody (P68431) for ChIP, CUT&Tag, WB, and IHC

Core Product Specifications and Parameters

Biological Background: Histone H3.1 Function and Localization

• Histone H3.1 is a core component of the nucleosome, the basic repeating unit of chromatin. Nucleosomes wrap and compact DNA, regulating access to the genetic information.
• As a key histone, H3 plays central roles in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled by a complex set of post-translational modifications (PTMs), collectively termed the 'histone code', which also involves nucleosome remodeling.
• The N-terminal tail of histone H3 is subject to a vast array of PTMs, including methylation, acetylation, phosphorylation, citrullination, and others. These marks are dynamic and orchestrate various chromatin-templated processes.
• Trimethylation of lysine 27 (H3K27me3) is a well-characterized repressive mark associated with gene silencing and heterochromatin formation, deposited primarily by the Polycomb repressive complex 2 (PRC2).
• Subcellularly, histone H3.1 is localized to the nucleus and associates with chromosomes.

Related references: UniProt P68431

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic trimethylated peptide spanning residues around K27. It is expected to specifically recognize the trimethylated form of histone H3 at lysine 27 and may not cross-react with unmodified or other methylated states. Consider validating specificity with peptide competition assays or appropriate positive/negative controls.
• For Western blot analysis, extraction of histones via acid-extraction protocols often improves signal and reduces background. Use a loading control such as total histone H3 or histone H4.
• For chromatin immunoprecipitation (ChIP) and CUT&Tag, optimize the antibody concentration and crosslinking conditions. The high specificity for H3K27me3 makes it suitable for mapping this repressive mark genome-wide.
• In immunohistochemistry (IHC-P), antigen retrieval (e.g., heat-induced epitope retrieval) may be required to expose the epitope. Validate the staining pattern in the relevant tissue and cell type.
• For immunofluorescence (IF/ICC), fixation and permeabilization conditions should be carefully chosen to preserve chromatin architecture and epitope accessibility.
• While this antibody is supplied for multiple applications, always pilot-test dilutions and conditions in your specific experimental system to ensure optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, supporting diagnostic manufacturers, research laboratories, and institutes with a full spectrum of reagents and technical services from concept to clinic. This TriMethyl-Histone H3-K27 monoclonal antibody (P68431) exemplifies our capability to supply high-quality, application-validated antibodies for epigenetic research and chromatin biology. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare-target raw materials, CamelBio provides reliable procurement solutions to accelerate your diagnostic and research programs.