TRIM5 Rabbit pAb for WB, ELISA - Q9C035

Core Product Specifications and Parameters

Biological Background: TRIM5 Function and Localization

• TRIM5 (Tripartite motif-containing protein 5), aliases RNF88 and TRIM5alpha, is a member of the TRIM protein family characterized by RING finger, B-box, and coiled-coil domains, and functions as a RING-type E3 ubiquitin ligase.
• It acts as a capsid-specific restriction factor that blocks retroviral infection early after entry but before reverse transcription, targeting viruses such as N-MLV, EIAV, SIVmac, FIV, and BIV. Related references: PMID:17156811
• Serves as a pattern recognition receptor for retroviral capsid lattices, triggering innate immune signaling via TAK1-dependent activation of NF-kappa-B and MAPK pathways.
• Regulates autophagy by activating BECN1 through dissociation from inhibitors BCL2 and TAB2, and by acting as a selective autophagy receptor that targets HIV-1 capsid protein p24 for autophagic degradation. Related references: PMID:25127057
• Localizes to both the cytoplasm and the nucleus.
• Post-translationally modified by acetylation and phosphorylation; the protein undergoes alternative splicing.
• Keywords include Antiviral defense, Innate immunity, Autophagy, Host-virus interaction, Zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 100-200) lies within the N-terminal region of TRIM5, which contains the RING and B-box domains. Verify expression in your target cell lines or tissues before use.
• For Western blotting, the predicted molecular weight is 56 kDa; however, due to post-translational modifications and isoforms, additional bands may be observed. Include appropriate loading controls.
• In ELISA applications, optimize coating antigen and antibody titration to achieve optimal signal-to-noise ratio.
• Confirm reactivity with human and mouse samples, as cross-reactivity is reported; if working with other species, validate independently.
• Consider using this antibody in combination with other tools for studying retroviral restriction, innate immune signaling, or autophagy pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibodies like this TRIM5 polyclonal antibody, which supports research in antiviral innate immunity and retroviral restriction. We offer optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or emerging targets to streamline your assay development and scaling needs.