TRIM47 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96LD4

Core Product Specifications and Parameters

Biological Background: TRIM47 Function and Localization

• TRIM47 (Tripartite motif-containing protein 47) is a member of the TRIM family, characterized by a RING finger domain, B-box, and coiled-coil region, functioning as an E3 ubiquitin-protein ligase.
• Mediates the ubiquitination and subsequent proteasomal degradation of CYLD, implicating it in NF-κB pathway regulation.
• Localizes to both the cytoplasm and nucleus, suggesting roles in multiple cellular compartments.
• Expressed at low levels in most tissues but shows higher expression in kidney tubular cells; notably overexpressed in astrocytoma tumor cells.
• Subject to post-translational modifications including acetylation, methylation, and phosphorylation (based on proteomic identifications).
• Contains a zinc-finger motif, indicating metal-binding properties; classified as a transferase in the ubiquitin conjugation pathway.
• Alternative splicing generates multiple transcript variants (Keywords: Alternative splicing).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to amino acids 356–530 of human TRIM47 (NP_258411.2). Users should ensure this epitope region is preserved in their target samples.
• For Western blotting, a band at approximately 70 kDa is expected; validate specificity using appropriate positive and negative controls in human, mouse, or rat lysates.
• In immunofluorescence/ICC, consider that TRIM47 localizes to both cytoplasm and nucleus; signal may be observed in both compartments.
• For ELISA, the antibody can be used as a detection reagent; titrate to determine optimal working concentration in the specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like TRIM47 involved in ubiquitin pathways and cancer, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our team can also assist in sourcing rare target raw materials to streamline your assay development.