TRIM27 Rabbit pAb - P14373

Core Product Specifications and Parameters

Biological Background: TRIM27 Function and Localization

• TRIM27 (Zinc finger protein RFP, RNF76) is a tripartite motif-containing E3 ubiquitin-protein ligase with RING finger, B-box, and coiled-coil domains, implicated in proliferation, innate immunity, apoptosis, and autophagy. Related references: PMID:22829933, PMID:24144979, PMID:29688809
• Negatively regulates NOD2 signaling by ubiquitinating NOD2 for proteasomal degradation. Together with MAGEL2, mediates Lys-63-linked ubiquitination of WASHC1 to promote endosomal actin assembly and retrograde transport. Related references: PMID:22829933, PMID:23452853
• Modulates apoptosis and antiviral signaling: ubiquitinates USP7 to regulate RIPK1 deubiquitination and TNF-induced apoptosis; cooperates with USP7 or PTPN11 to ubiquitinate and degrade TBK1, suppressing antiviral responses. Related references: PMID:24144979, PMID:26358190, PMID:29688809
• Regulates autophagy: negatively regulates basal autophagy by ubiquitinating ULK1; during starvation, ubiquitinates STK38L, leading to ULK1 phosphorylation and degradation to restrain autophagy. Facilitates mitophagy by stabilizing TBK1. Related references: PMID:35670107, PMID:36111389
• Has transcriptional repressor activity in cooperation with EPC1, and induces apoptosis via JNK/p38 and caspase-3-like activity.
• Localizes to nucleus, cytoplasm, PML bodies, early endosomes, and mitochondria; expressed in testis within seminiferous tubules. Functions as a proto-oncogene and DNA-binding protein.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 1-200 amino acids of human TRIM27, encompassing the RING finger domain critical for its E3 ubiquitin ligase activity. This design may support detection of the functional domain.
• For IHC-P, since TRIM27 is expressed in testis within seminiferous tubules, testis tissue sections could be a suitable positive control. Validate staining with appropriate negative controls.
• In ELISA applications, the polyclonal nature of this antibody may provide sensitive detection of native and recombinant TRIM27. Confirm cross-reactivity in mouse and rat lysates if these species are used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials, including this anti-TRIM27 polyclonal antibody for studying ubiquitination and autophagy pathways. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development from concept to clinic.