TREML1 Rabbit Polyclonal Antibody for WB, ELISA - Q86YW5

Core Product Specifications and Parameters

Biological Background: TREML1 Function and Localization

• TREML1, also known as Triggering receptor expressed on myeloid cells-like protein 1 (TLT-1), is a cell surface receptor belonging to the immunoglobulin superfamily.
• It functions in the innate and adaptive immune response, though its precise role remains under investigation.
• TREML1 is a transmembrane protein localized primarily at the cell membrane, but also detected in the cytoplasm.
• The protein contains an extracellular immunoglobulin domain, a transmembrane helix, and a cytoplasmic tail with potential phosphorylation sites, suggesting involvement in signal transduction.
• Post-translational modifications include palmitoylation and disulfide bond formation, which may influence its membrane anchoring and stability.
• TREML1 is detected in platelets, monocytic leukemia, and T-cell leukemia, indicating a role in hematopoietic cells.
• Alternative splicing generates multiple isoforms, potentially diversifying its function.
• The 3D structure has been resolved, providing insights into its receptor architecture.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 16-162), likely yielding antibodies that recognize the native protein in membrane extracts.
• For Western blotting, the predicted molecular weight is 33 kDa, but glycosylation may shift the observed band; validate using positive control lysates from human platelets or relevant cell lines.
• For ELISA, this polyclonal antibody can be used as capture or detection reagent; pairing with a monoclonal may improve specificity. Titrate to optimize assay conditions.
• Cross-reactivity with mouse and rat has been noted; however, sequence alignment should be checked for each application to ensure target recognition in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized polyclonal antibodies like this anti-TREML1 antibody, and bulk ancillary reagents to support diagnostic development in immunology and receptor research. With services spanning concept to clinic, we help streamline procurement of rare target reagents and provide technical consultation to accelerate your assay development.