TRAIL/TNFSF10 Rabbit mAb - P50591

Core Product Specifications and Parameters

Biological Background: TRAIL/TNFSF10 Function and Localization

• TRAIL (TNF-related apoptosis-inducing ligand), also known as Apo-2L, CD253, or TL2, is a member of the TNF superfamily encoded by the TNFSF10 gene.
• It binds to death receptors TNFRSF10A/TRAILR1 and TNFRSF10B/TRAILR2, and decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG. Related references: PMID:10549288 PMID:26457518
• Binding to death receptors triggers apoptosis; decoy receptor interaction may modulate its apoptotic activity and serve as a regulatory mechanism.
• It is expressed as a type II transmembrane protein but can be cleaved to release a soluble ligand; localized to the cell membrane and also secreted.
• Widely expressed in tissues, with highest levels in spleen, lung, and prostate.
• The protein undergoes alternative splicing, phosphorylation, and zinc binding, contributing to its regulation.
• UniProt lists a molecular weight of approximately 32.5 kDa for the full-length protein; additional isoforms or cleaved products may be detected at lower molecular weights, such as 12 kDa.

Experimental Guidance and Technical Tips

• In western blotting, consider using a reducing SDS-PAGE to detect both the full-length (33 kDa) and cleaved (12 kDa) forms of TRAIL; verify band patterns against appropriate positive controls.
• For IHC-P, antigen retrieval may be required; optimize retrieval conditions and primary antibody dilution using tissue samples known to express TRAIL.
• When performing IF/ICC, include suitable permeabilization and blocking steps; co-staining with organelle markers can help confirm localization.
• Confirm cross-reactivity in mouse and rat model systems by testing on relevant cell or tissue lysates, as variability may exist between species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop platform for IVD raw materials. This TRAIL/TNFSF10 rabbit monoclonal antibody supports apoptosis and cancer research, and we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing to accelerate your assay development and scale-up.