TRAF4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BUZ4

Core Product Specifications and Parameters

Biological Background: TRAF4 Function and Localization

• Acts as an adapter protein with E3 ligase activity involved in many biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation, and apoptosis. Related references: PMID:30352854, PMID:31076633, PMID:32268273
• Promotes EGFR-mediated signaling by facilitating EGFR dimerization and downstream AKT activation, thereby promoting cell proliferation. Related references: PMID:30352854
• Ubiquitinates SMURF2 via 'Lys-48'-linked chains, leading to SMURF2 proteasomal degradation and promoting osteogenic differentiation. Related references: PMID:31076633
• Catalyzes 'Lys-63'-linked ubiquitination of CHK1, activating cell cycle arrest and DNA repair pathways. Related references: PMID:32357935
• Mediates an atypical 'Lys-29'-linked ubiquitination at the C-terminus of IRS1, which is essential for insulin-like growth factor (IGF) signal transduction. Related references: PMID:33991522
• Inhibits adipogenic differentiation by activating pyruvate kinase PKM and the beta-catenin signaling pathway. Related references: PMID:32268273
• Localizes to the cytoplasm, nucleus, perinuclear region, tight junctions, cell membrane, and cytoskeleton.
• Highly expressed in epithelial cells of the thymus, dendritic cells of lymph node, and the basal cell layer of various epithelia including epidermis, respiratory tract, and salivary gland.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 251–470, which encompasses part of the TRAF domain; the polyclonal nature of this antibody suggests recognition of multiple epitopes and likely detection of full-length TRAF4 (~54 kDa) in western blot.
• For WB, consider loading 20–30 µg of total protein lysate from human, mouse, or rat cell lines or tissues; optimize detection conditions for your specific samples.
• In IHC-P and IF/ICC, fixation and permeabilization protocols should be optimized; the antibody may detect both cytoplasmic and nuclear TRAF4 depending on context.
• Cross-reactivity with mouse and rat indicates conserved epitopes, but validation in the relevant species and application is recommended.
• The antibody is suitable for ELISA; explore its utility for capture or detection in sandwich immunoassays with matched pairs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and targeted support for rare targets. This TRAF4-specific antibody supports research in cell signaling, oncology, and immunology, and CamelBio can assist with further reagent sourcing and technical consultation to accelerate your diagnostic development from concept to clinic.