TPM3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P06753

Core Product Specifications and Parameters

Biological Background: Tropomyosin alpha-3 chain Function and Localization

• Tropomyosin alpha-3 chain (TPM3), also known as gamma-tropomyosin or tropomyosin-3, is a member of the tropomyosin family that binds to actin filaments in both muscle and non-muscle cells.
• In striated muscle, TPM3 plays a central role in calcium-dependent regulation of contraction by interacting with the troponin complex, while in smooth muscle it regulates contraction via caldesmon.
• Multiple isoforms are generated through alternative splicing, contributing to functional diversity across tissues and developmental stages.
• Subcellularly, TPM3 localizes to the cytoplasm, predominantly associated with the cytoskeleton, where it stabilizes actin filaments.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its actin-binding properties and cytoskeletal dynamics.
• TPM3 is implicated in human diseases; chromosomal rearrangements involving TPM3 are associated with certain cancers, and mutations cause nemaline myopathy, a congenital muscle disorder.
• Non-muscle isoforms are essential for maintaining cytoskeletal integrity and regulating cell motility.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-285 of human TPM3, a region conserved across species; consider testing cross-reactivity with other samples if needed.
• For Western blot, the predicted molecular weight is ~33 kDa; ensure adequate denaturation and gel resolution to distinguish TPM3 from closely related tropomyosin isoforms.
• In immunofluorescence, co-staining with actin markers can help confirm specificity; optimize fixation conditions to preserve cytoskeletal architecture.
• For ELISA, validate antibody performance as a capture or detection reagent using recombinant TPM3 or relevant biological matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers this anti-TPM3 polyclonal antibody as part of a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents for cytoskeleton and muscle research. With expert sourcing capabilities for rare targets and flexible supply models, CamelBio supports diagnostic developers and labs from concept to clinic—ask our team about customized raw-material solutions.