TP53BP1 Rabbit pAb - Q12888

Core Product Specifications and Parameters

Biological Background: TP53BP1 Function and Localization

• TP53BP1 (53BP1) is a double-strand break (DSB) repair protein that plays a central role in DNA damage signaling, telomere maintenance, and immunoglobulin class-switch recombination (CSR). Related references: PMID:12364621, PMID:17190600, PMID:21144835
• It promotes non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracts BRCA1-dependent homologous recombination, preserving genome stability. Related references: PMID:22553214, PMID:23333306, PMID:23727112
• Following DNA damage, ATM-mediated phosphorylation of 53BP1 triggers its interaction with RIF1 and release from the inhibitory protein TIRR/NUDT16L1, enabling recruitment to damage sites. Related references: PMID:28241136
• 53BP1 is targeted to DSBs through bivalent recognition of histone marks: monoubiquitinated H2A at Lys-15 (H2AK15Ub) and dimethylated H4 at Lys-20 (H4K20me2). Related references: PMID:17190600, PMID:23760478, PMID:27153538
• Required for CSR during antibody genesis, it participates in the repair and correct orientation of broken DNA ends. Related references: PMID:23345425
• 53BP1 also promotes NHEJ of dysfunctional telomeres through interaction with PAXIP1, contributing to telomere capping. Related references: PMID:23727112
• Subcellular localization is predominantly nuclear, with association to chromosomes and enrichment at centromeres and kinetochores.
• The protein undergoes extensive post-translational modifications, including phosphorylation, methylation, isopeptide bond formation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 895-1031 of human TP53BP1. It is designed to target the central region and may detect full-length as well as certain splice isoforms; researchers should verify specificity in their model system.
• In Western blot, the predicted molecular weight is ~214 kDa. Due to the large size, consider using a low-percentage gel and extended transfer conditions for optimal detection.
• For immunohistochemistry (IHC-P), standard antigen retrieval methods are recommended; titration experiments can help establish the optimal working dilution.
• Cross-reactivity is reported for human, mouse, and rat. Verify performance in other species prior to use.
• This antibody is suitable for ELISA; sandwich pairing compatibility should be assessed empirically if used in combination with other antibodies.
• As a polyclonal antibody, lot-to-lot variability may occur; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers, supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This TP53BP1 antibody supports research into DNA damage response and class-switch recombination, and CamelBio can assist with sourcing rare-target reagents and custom development needs. From concept to clinic, CamelBio streamlines procurement of critical raw materials for diagnostic development.