Topoisomerase II alpha/TOP2A Rabbit mAb for WB, IF/ICC, IF-P, IHC-P, ELISA - P11388

Core Product Specifications and Parameters

Biological Background: Topoisomerase II alpha Function and Localization

• DNA topoisomerase 2-alpha (TOP2A) is a key decatenating enzyme that alters DNA topology by generating transient double-stranded breaks, passing an intact strand through the break, and religating it. Related references: PMID:17567603 PMID:18790802 PMID:22013166
• This enzyme is essential for resolving DNA tangles and supercoils that arise during replication, transcription, chromosome segregation, and other processes requiring topological manipulation of DNA.
• TOP2A is a member of the type II topoisomerase family and exists as a homodimer; it is also known as DNA topoisomerase II alpha isozyme.
• The protein localizes to the cytoplasm, nucleoplasm, and nucleolus, consistent with its roles in nuclear DNA metabolism and mitotic chromosome organization.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, as indicated by UniProt keywords; these modifications may regulate enzyme activity, stability, and cellular localization.
• TOP2A is widely expressed in tissues such as tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain, and lung. Elevated expression is observed in high-grade lymphomas, squamous cell lung tumors, and seminomas.
• It may also play a role in circadian rhythm regulation by influencing the period length of BMAL1 transcriptional oscillation (By similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 1431-1531 within the C-terminal region of human TOP2A, a region that may confer isoform specificity. Consider verifying specificity via knockdown or knockout controls.
• For Western blot, the predicted molecular weight of TOP2A is approximately 174 kDa. Use a low-percentage acrylamide gel (e.g., 6-8%) and extended transfer times for efficient blotting of this high molecular weight protein.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), nuclear and nucleolar staining patterns are expected. Validate by comparing with known positive tissues such as tonsil, spleen, or high-grade lymphomas.
• For ELISA, ensure the coating antigen presents the epitope region appropriately; peptide competition assays can help confirm binding specificity.
• As a recombinant rabbit monoclonal antibody, it offers high affinity and reproducibility; however, optimal working dilutions should be determined empirically for each assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw material solutions for diagnostic and research applications, including validated antibody pairs, optimized recombinant monoclonal antibodies, and bulk ancillary reagents. For studies on DNA topology and topoisomerase biology, CamelBio can source rare-target raw materials and offer technical consulting to support your assay development from concept to clinic.