TOMM7 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9P0U1

Core Product Specifications and Parameters

Biological Background: TOMM7 Function and Localization

• Mitochondrial import receptor subunit TOM7 homolog (TOMM7, also known as TOM7) is a core component of the translocase of the outer membrane of mitochondria (TOM) complex, essential for recognition and translocation of cytosolically synthesized mitochondrial preproteins.
• TOMM7 participates in the PINK1-dependent mitophagy pathway: at depolarized mitochondria, the TOM complex associates with VDAC2 and PINK1 kinase, stabilizing PINK1 on the outer membrane and triggering downstream recruitment of the E3 ubiquitin ligase PRKN (Parkin). Related references: PMID:40080546
• The TOMM7 gene encodes a small 6 kDa protein with a single transmembrane helix, classified under mitochondrial outer membrane transport proteins.
• Subcellular localization is strictly the mitochondrial outer membrane, consistent with its role in preprotein import and signaling.
• Post‑translational modifications and disease associations are still under investigation; however, the UniProt record lists ‘Disease variant’ as a keyword, hinting at potential links to human pathologies.

Experimental Guidance and Technical Tips

• The immunogen was designed against a recombinant protein spanning amino acids 37–55 of human TOMM7, which corresponds to a highly conserved region. Researchers may consider this epitope accessible for detection in native and denatured samples.
• Recommended applications (WB, IHC-P, IF/ICC, ELISA) are supported by use of a recombinant immunogen; however, optimal working dilutions and conditions should be determined empirically in the relevant sample system.
• For mitochondrial import studies, proper subcellular fractionation or inclusion of mitochondrial markers is advised to confirm specific localization.
• TOMM7’s small molecular weight (6 kDa) may require special attention during SDS‑PAGE (high‑percentage gels, short transfer times) and blotting to prevent loss or poor resolution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This TOMM7 antibody exemplifies our ability to provide validated monoclonal antibodies for mitochondrial biology targets, and we can additionally supply matched antibody pairs, optimized poly‑ or monoclonal reagents, bulk ancillary materials, and assist in sourcing rare‑target raw materials to accelerate your assay development.