TOMM34 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15785

Core Product Specifications and Parameters

Biological Background: TOMM34 Function and Localization

• TOMM34 is a mitochondrial import receptor subunit, also known as TOM34, URCC3, or HTOM34P.
• Functions in the import of cytosolically synthesized preproteins into mitochondria by binding the mature portion of precursor proteins and exhibiting weak ATPase activity.
• Acts as a chaperone-like protein, keeping newly synthesized precursors in an unfolded, import-competent state.
• Localizes to the cytoplasm and mitochondrion outer membrane, as part of the TOM (translocase of the outer membrane) complex.
• Contains TPR (tetratricopeptide repeat) domains, which mediate protein-protein interactions, and is subject to phosphorylation and ubiquitin-like conjugation.
• Ubiquitously expressed in human tissues, indicating a housekeeping role in mitochondrial biogenesis.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full length of TOMM34 (aa 1-309), likely providing broad epitope coverage for detection by Western blot; validate in your sample system.
• For IF/ICC, consider optimizing fixation and permeabilization protocols to visualize the cytoplasmic and mitochondrial outer membrane pools of TOMM34.
• Confirm antibody specificity using siRNA-mediated knockdown or TOMM34 knockout cell lines as negative controls.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for multi-species studies; verify performance in the relevant model organism.
• For ELISA applications, titrate coating antigen and detection conditions empirically to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD development with a comprehensive portfolio of raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-TOMM34 pAb, and bulk ancillary reagents. Whether your focus is mitochondrial biology or any other target, our sourcing services help bridge the gap from concept to clinic.