TNNT3 Rabbit pAb - P45378

Core Product Specifications and Parameters

Biological Background: TNNT3 Function and Localization

• The TNNT3 gene encodes Troponin T, fast skeletal muscle, also known as beta-TnTF, which is a critical regulatory protein in striated muscles.
• Functionally, TNNT3 serves as the tropomyosin-binding subunit of the troponin complex, conferring calcium sensitivity to actomyosin ATPase activity essential for muscle contraction.
• In terms of tissue expression, TNNT3 is specifically detected in fetal and adult fast skeletal muscles, with a notably higher expression level in fetal compared to adult muscle.
• Post-translational modifications such as acetylation and phosphorylation have been identified, suggesting regulatory roles in modulating its function.
• The gene is subject to alternative splicing, potentially generating multiple isoforms that contribute to functional diversity and fine-tuning of muscle contractility.
• Mutations in TNNT3 are associated with the disease variant keyword in UniProt, indicating its involvement in muscle-related pathologies, though specific disorders may require further investigation.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 147-256 of human TNNT3, corresponding to a region within the protein. In Western blotting, a band at approximately 32 kDa is typically expected; however, optimization of loading amounts and detection conditions is recommended.
• Given its cross-reactivity with mouse and rat, this antibody can be explored for studies in rodent models, but validation in the specific experimental system is advised to confirm specificity and signal-to-noise ratio.
• For ELISA applications, the polyclonal nature may provide robust signal; nevertheless, pairing with a matched detection antibody or assessing the dynamic range is advisable for reproducible quantitative assays.
• Consider using fast skeletal muscle lysates as positive controls to verify reactivity, and include appropriate negative controls (e.g., non-muscle tissues or knockout samples) to assess background.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we specialize in providing a comprehensive suite of IVD raw materials and technical support to accelerate your diagnostic and research workflows. This anti-TNNT3 polyclonal antibody is part of our extensive portfolio, supporting skeletal muscle and troponin-related studies. From validated antibody pairs and optimized polyclonal reagents to bulk ancillary materials and rare target sourcing, CamelBio is your trusted partner for all IVD raw material needs, from concept to clinic.