TMEM44 Rabbit pAb - Q2T9K0

Core Product Specifications and Parameters

Biological Background: TMEM44 Function and Localization

• TMEM44, also known as Transmembrane protein 44, is encoded by the TMEM44 gene in humans.
• It is predicted to be a multi-pass membrane protein based on the presence of several transmembrane helices.
• Subcellular localization is at the membrane according to UniProt annotation.
• The protein undergoes alternative splicing, producing multiple isoforms with potentially distinct functions.
• It has been identified as a phosphoprotein in high-throughput proteomic studies.
• The canonical isoform has a calculated molecular weight of approximately 52 kDa.
• TMEM44 is included in the UniProt reference proteome set, indicating its curation quality.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 110-260 of human TMEM44 (NP_001159777.1), a region that may be conserved across species, as suggested by the observed cross-reactivity with mouse and rat.
• For Western blot (WB), consider using whole-cell lysates or membrane fractions from human, mouse, or rat tissues/cells as positive controls, and validate the expected band size (~52 kDa).
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be required to access this membrane protein; optimize fixation and permeabilization conditions for your cell type.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody may improve specificity.
• As a polyclonal antibody, lot-to-lot variability may occur; we recommend validating each new lot in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, from concept to clinic. For target proteins like TMEM44, we offer validated polyclonal antibodies and can assist in sourcing bulk reagents, antibody pairs, and rare-target materials to accelerate your assay development. As your strategic sourcing partner, we support the entire IVD development cycle with a broad catalog of antibodies, antigens, and ancillary reagents tailored to your needs.