TMEM43 Rabbit Polyclonal Antibody for WB, ELISA - Q9BTV4

Core Product Specifications and Parameters

Biological Background: TMEM43 Function and Localization

• TMEM43, also known as LUMA, is a transmembrane protein required for maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane and retaining emerin (by similarity).
• It localizes to the endoplasmic reticulum membrane, nuclear inner membrane, and cell membrane, reflecting its multifunctional roles in cellular signaling and structural integrity.
• TMEM43 modulates innate immune signaling through the cGAS-STING pathway by interacting with RNF26, highlighting its role in antiviral responses. Related references: PMID:32614325
• It functions as a critical signaling component in mediating NF-kappa-B activation by acting downstream of EGFR and upstream of CARD10, linking growth factor signaling to inflammatory responses. Related references: PMID:27991920
• Contributes to passive conductance current in cochlear glia-like supporting cells, mediated by gap junctions and necessary for hearing and speech discrimination. Related references: PMID:34050020
• Highest expression in placenta, with lower levels in heart, ovary, spleen, small intestine, thymus, prostate, and testis, suggesting tissue-specific functions in development and homeostasis.
• Post-translational modifications include acetylation, and mutations are associated with diseases such as cardiomyopathy, Emery-Dreifuss muscular dystrophy, deafness, and neuropathy.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment (amino acids 80–310) expressed in a heterologous system; consider epitope accessibility and potential differences in endogenous vs. recombinant protein conformation when designing experiments.
• For Western blot, the predicted molecular weight is approximately 45 kDa; validate detection in relevant cell lines or tissues (e.g., placental, cardiac) and include appropriate positive and negative controls.
• For ELISA applications, optimize coating and detection conditions with purified protein or cell lysates; pairing with a matched detection antibody may improve sensitivity in sandwich assays.
• Reported cross-reactivity with human and mouse; verify reactivity in your sample system before large-scale studies, especially if using tissues or cell lines from other species.
• The antibody is shipped on ice bag; upon receipt, store at the recommended temperature to maintain stability and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with a seamless supply chain for specialized raw materials, including validated antibodies for nuclear envelope and innate immunity targets like TMEM43. Our portfolio offers ready-to-use polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or hard-to-find raw materials. Contact us to discuss your project needs—from single reagents to customized antibody pairs and comprehensive technical support.