TMEM184A Rabbit Polyclonal Antibody for WB and ELISA - Q6ZMB5

Core Product Specifications and Parameters

Biological Background: TMEM184A Function and Localization

• TMEM184A (Transmembrane protein 184A), also known as SDMG1, is a multi-pass transmembrane protein encoded by the TMEM184A gene in humans.
• It functions as a heparin receptor in vascular cells, mediating cellular responses to heparin (By similarity).
• TMEM184A may be involved in vesicle transport processes, particularly in exocrine cells and Sertoli cells (By similarity).
• The protein localizes to the cell membrane, cytoplasm, perinuclear region, and membranes of cytoplasmic vesicles, including early endosomes and secretory vesicles.
• At the protein level, TMEM184A is expressed in vascular cells, suggesting tissue-specific roles in the vasculature.
• UniProt annotations highlight its transmembrane topology and presence in cytoplasmic vesicles, consistent with a role in endocytosis and intracellular trafficking.
• The molecular weight of the full-length protein is approximately 46 kDa (calculated from sequence as ~45.8 kDa).

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 324–413 of human TMEM184A. Based on predicted topology, this region may be intracellular; consider this when designing detection protocols that require antigen accessibility.
• For Western blot (WB), use appropriate positive controls such as lysates from vascular cell lines or tissues expressing TMEM184A. Validate the expected band size of ~46 kDa.
• This antibody cross-reacts with mouse and rat TMEM184A, offering flexibility for studies in rodent models. However, confirm reactivity experimentally in your specific sample system.
• In ELISA applications, consider using recombinant TMEM184A protein or peptide fragments encompassing the immunogen region as coating antigens for optimal detection.
• As a polyclonal antibody, it recognizes multiple epitopes, which may increase sensitivity but also requires careful validation to confirm specificity in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, offering a comprehensive range of antibodies, antigens, and ancillary reagents to support diagnostic manufacturers and research laboratories. This anti-TMEM184A rabbit polyclonal antibody is part of our extensive catalog for vascular biology and cell trafficking research, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk quantities tailored to your assay development needs. Through our one-stop sourcing bridge, we streamline procurement from concept to clinic, enabling you to secure rare target reagents and scale your projects efficiently.