TMEM165 Rabbit Polyclonal Antibody for WB and ELISA - Q9HC07

Core Product Specifications and Parameters

Biological Background: TMEM165 Function and Localization

• TMEM165 is a putative divalent cation/proton antiporter that mediates the exchange of calcium (Ca²⁺) or manganese (Mn²⁺) ions for protons across the Golgi membrane, providing essential cofactors to resident Golgi enzymes (PubMed:22683087, PubMed:23569283, PubMed:27008884). Related references: PMID:22683087 PMID:23569283 PMID:27008884
• The protein contributes to the maintenance of an acidic luminal pH in the Golgi, which is critical for proper secretory pathway function.
• TMEM165 is encoded by the TMEM165 gene, also known as TPARL, and alternative splicing generates multiple transcript variants (Keywords: Alternative splicing).
• It is localized to the Golgi apparatus membrane (SUBCELLULAR LOCATION: Golgi apparatus membrane).
• In mammary epithelial cells, TMEM165 promotes Ca²⁺ storage within the Golgi lumen, facilitating its secretion into milk (By similarity).
• Ubiquitously expressed across tissues (TISSUE SPECIFICITY).
• Mutations in TMEM165 are associated with congenital disorder of glycosylation type IIk (CDG2K), highlighting its role in glycosylation processes (Keywords: Congenital disorder of glycosylation, Disease variant).
• The protein contains predicted transmembrane helices and a signal sequence, consistent with its membrane localization (Keywords: Signal, Transmembrane helix).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 172-230 of human TMEM165, suggesting the antibody may recognize an epitope within this region.
• For Western blot (WB), the expected molecular weight is approximately 35 kDa; consider using human cell lines or tissues as positive controls.
• For ELISA, this antibody may be suitable for detecting recombinant or native TMEM165; optimal working dilutions should be empirically determined.
• Since the antibody shows cross-reactivity with human TMEM165, validate specificity and sensitivity in the relevant biological matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This TMEM165 rabbit polyclonal antibody targets a key Golgi ion transporter implicated in glycosylation disorders, supporting your research into congenital disorders of glycosylation and metal ion homeostasis. Beyond antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development workflows.