TM9SF1 Rabbit Polyclonal Antibody for WB and ELISA - O15321

Core Product Specifications and Parameters

Biological Background: TM9SF1 Function and Localization

• TM9SF1, also known as MP70 or hMP70, is a member of the transmembrane 9 superfamily, encoded by the TM9SF1 gene on human chromosome 14.
• It is an integral multi-pass membrane protein featuring a signal peptide and several transmembrane helices, with a predicted molecular weight of approximately 69 kDa.
• TM9SF1 is primarily localized to the membranes of lysosomes and autophagosomes, as well as to cytoplasmic vesicles, consistent with its role in membrane trafficking.
• The protein plays an essential role in autophagy, the conserved cellular process responsible for the degradation and recycling of cytoplasmic components.
• Post-translational modifications include glycosylation, as evidenced by its classification as a glycoprotein in UniProt, and the gene undergoes alternative splicing.
• Tissue expression is broad, with relatively high levels detected in lung, pancreas, kidney, liver, and placenta; lower expression is observed in skeletal muscle, heart, and brain.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the expected band size is approximately 69 kDa; consider using a gradient gel and adequate transfer time to ensure proper resolution and transfer of this transmembrane protein.
• Given the antibody's immunogen (recombinant fragment covering amino acids 28–230), it may recognize linear epitopes; for ELISA, direct coating of the immunogen or denatured protein samples may yield optimal detection.
• The antibody cross-reacts with both human and mouse TM9SF1, making it a candidate for studies involving mouse models; however, confirm species-specific expression patterns and antibody reactivity in your system.
• As TM9SF1 is an autophagy-related membrane protein, enrichment techniques such as membrane fractionation or autophagosome isolation may enhance detection in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your dedicated procurement partner, connecting diagnostic manufacturers, labs, and research institutes with high-quality IVD raw materials. Whether you need validated antibody pairs, optimized polyclonal antibodies like this TM9SF1 reagent, or bulk ancillary reagents, CamelBio offers reliable supply chain solutions. For targets involved in autophagy and membrane biology, we can also assist in sourcing rare raw materials to streamline your assay development from concept to clinic.