TM4SF19 Rabbit pAb - Q96DZ7

Core Product Specifications and Parameters

Biological Background: TM4SF19 Function and Localization

• Encoded by the TM4SF19 (OCTM4) gene in humans, Transmembrane 4 L6 family member 19 (TM4SF19) is a 22 kDa multi-pass membrane protein belonging to the tetraspanin superfamily.
• TM4SF19 negatively regulates vacuolar (H+)-ATPase (V-ATPase) activity by binding to V0 complex subunits, thereby inhibiting V1-V0 holoenzyme assembly and acidification of intracellular compartments.
• This regulatory function is essential for osteoclast differentiation: TM4SF19 is required for multinucleation, a critical step in the maturation of bone-resorbing osteoclasts.
• At the subcellular level, TM4SF19 localizes primarily to the lysosome membrane and also distributes to the cytoplasm, cytoskeleton, and cell projections such as filopodia.
• Tissue expression is selective; in adipose tissue, TM4SF19 is expressed specifically by macrophages, suggesting roles in immune cell function within metabolic microenvironments.
• Post-translational modifications include N-linked glycosylation, and the protein contains several transmembrane helices consistent with its membrane topology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 50-150) of human TM4SF19, which is conserved in mouse. This design supports the observed cross-reactivity and can be suitable for detecting both endogenous and exogenous TM4SF19.
• For Western blotting, expect a band near 22 kDa under reducing conditions. Consider using appropriate lysis buffers to solubilize membrane proteins effectively.
• In immunofluorescence (IF-P), signal may be observed at lysosomes, filopodia, or cytoplasmic puncta, depending on cell type and fixation/permeabilization conditions.
• For ELISA applications, titrate the antibody and validate with a suitable antigen (e.g., recombinant protein or peptide) to determine optimal working concentration.
• Given the role of TM4SF19 in osteoclast biology, primary osteoclast cultures or macrophage cell lines (e.g., RAW 264.7) differentiated with RANKL could serve as relevant positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This TM4SF19 antibody supports investigations into lysosomal regulation and osteoclast function, opening possibilities for metabolic bone disease research. For projects requiring rare-target reagents or scaled-up production, CamelBio offers dedicated sourcing assistance and technical consultation to accelerate your development pipeline.