TLR2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60603

Core Product Specifications and Parameters

Biological Background: TLR2 Function and Localization

• TLR2, also known as Toll/interleukin-1 receptor-like protein 4 (TIL4) or CD282, is a member of the Toll-like receptor family that plays a key role in innate immunity.
• It acts as a pattern recognition receptor, forming heterodimers with TLR1 or TLR6 to recognize bacterial lipoproteins and lipopeptides, thereby initiating signaling cascades (PubMed:17889651, PubMed:21078852). Related references: PMID:17889651 PMID:21078852
• Upon ligand binding, TLR2 recruits adaptor proteins MYD88 and TRAF6, leading to activation of NF-kappa-B and production of pro-inflammatory cytokines (PubMed:10426995, PubMed:10426996). Related references: PMID:10426995 PMID:10426996
• TLR2 recognizes a broad range of microbial components including MALP-2, STF, PSM, and OspA-L from bacteria such as Mycoplasma and Borrelia (PubMed:11441107). Related references: PMID:11441107
• It mediates responses to Mycobacterium tuberculosis products like lipoproteins LprA, LprG, LpqH, PstS1, and HSP70, contributing to host-pathogen interactions (PubMed:19362712, PubMed:15809303). Related references: PMID:19362712 PMID:15809303
• Subcellular localization includes the plasma membrane, cytoplasmic vesicle membrane, phagosome membrane, and membrane rafts, with clustering in lipid rafts upon activation (PubMed:16880211). Related references: PMID:16880211
• TLR2 is highly expressed in peripheral blood leukocytes, particularly monocytes, as well as in bone marrow, lymph node, spleen, and lung, consistent with its immune surveillance role.
• The protein features leucine-rich repeats (LRRs) for ligand recognition, a transmembrane domain, and a TIR cytoplasmic domain; it undergoes glycosylation and disulfide bond formation, with a predicted molecular weight of ~90 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, consider the predicted molecular weight of ~90 kDa when analyzing TLR2 expression.
• For IHC-P, antigen retrieval may improve detection; validate in relevant positive controls like spleen or lymph node.
• Polyclonal antibodies may show lot-to-lot variation; include a standardized positive control in each experiment.
• Cross-reactivity with mouse and rat is noted; verify target specificity in the species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibodies, antigen pairs, and ancillary reagents. Our TLR2 Rabbit pAb supports research in innate immunity and infectious disease, and we offer bulk purchasing options and customized sourcing for rare targets. From concept to clinic, CamelBio helps streamline your diagnostic development.