TIMMDC1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NPL8

Core Product Specifications and Parameters

Biological Background: TIMMDC1 Function and Localization

• TIMMDC1 (Translocase of inner mitochondrial membrane domain-containing protein 1), also known as C3orf1 or MC1DN31, is a mitochondrial chaperone protein crucial for complex I assembly.
• It facilitates the construction of the membrane arm of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I), a key component of the electron transport chain.
• The protein is localized to the mitochondrial membrane and features transmembrane helices embedded in the inner membrane.
• TIMMDC1 is ubiquitously expressed but shows enhanced expression in heart and skeletal muscle, reflecting high metabolic demand.
• Mutations in the TIMMDC1 gene are linked to primary mitochondrial disease (MC1DN31), underscoring its pathological significance.
• The protein is subject to phosphorylation and has been identified through comprehensive proteomics analyses.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody targets a ~32 kDa band; consider using mitochondrial-enriched lysates from heart or skeletal muscle to improve detection.
• In immunohistochemistry (IHC-P), antigen retrieval methods may enhance signal; validate the antibody on known positive tissues such as heart or skeletal muscle sections.
• When performing ELISA, use the recombinant immunogen (aa 206-285) as a positive control to establish assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This TIMMDC1 antibody is ideally suited for studies on mitochondrial complex I assembly and mitochondrial disorders. CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic and research workflows.