TIMM13 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y5L4

Core Product Specifications and Parameters

Biological Background: TIMM13 Function and Localization

• TIMM13 encodes the mitochondrial import inner membrane translocase subunit Tim13, a small ~10.5 kDa protein of the mitochondrial intermembrane space.
• It functions as a chaperone, participating in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. It also facilitates the transfer of β-barrel precursors from the TOM complex to the SAM complex.
• Tim13 forms a heterohexameric complex with Tim8 (TIMM8), which specifically mediates the import of substrates such as TIMM23 and the mitochondrial carriers SLC25A12 and SLC25A13.
• Subcellularly, TIMM13 localizes to the mitochondrial inner membrane, facing the intermembrane space, consistent with its role in protein translocation.
• It is ubiquitously expressed, with highest levels in heart, kidney, liver, and skeletal muscle, reflecting the high mitochondrial activity in these tissues.
• Post-translational modifications include acetylation, disulfide bond formation, and phosphorylation; it also binds zinc, which is critical for its structural stability.
• Keywords associated with TIMM13 include chaperone, protein transport, mitochondrial inner membrane, translocation, and metal-binding.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein encompassing amino acids 1–95 of human TIMM13 (NP_036590.1). Because this polyclonal antibody was raised against the full-length protein, it may recognize multiple epitopes; consider verifying specificity via knockdown or knockout experiments.
• This antibody is recommended for Western blot (WB) and ELISA. For WB, the predicted molecular weight of TIMM13 is ~11 kDa; a band near this size is expected. Enriching mitochondrial fractions may improve detection sensitivity.
• Cross-reactivity is expected with human, mouse, and rat based on sequence homology, but individual sample validation is advised.
• When performing ELISA, the polyclonal nature of this antibody may provide robust signal; however, appropriate controls should be included to confirm target binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies like this anti-TIMM13 antibody, and bulk ancillary reagents. Our supply chain ensures timely access to high-quality materials for mitochondrial research, protein import studies, and diagnostic development. Whether you require rare target antibodies or comprehensive technical support, we bridge the gap from concept to clinic.