TIAM1 Rabbit pAb for WB, IHC-P, ELISA - Q13009

Core Product Specifications and Parameters

Biological Background: TIAM1 Function and Localization

• TIAM1 is a Rho guanine nucleotide exchange factor (GEF) that specifically activates RAC1, CDC42, and to a lesser extent RHOA, linking extracellular signals to cytoskeletal reorganization.
• It plays a critical role in regulating cell adhesion and migration through the downstream signaling of these Rho GTPases.
• TIAM1 localizes at cell junctions and the cell membrane, where it facilitates localized actin dynamics.
• The protein is expressed in a wide range of tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas, and carcinomas, suggesting a role in cancer cell invasion.
• Alternative splicing produces multiple isoforms, and the protein is subject to post-translational modifications such as phosphorylation and ubiquitination.
• According to UniProt, TIAM1 is also annotated with keywords like 3D-structure, lipid-binding, and myristoylation, indicating its structural and membrane-targeting features.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 1392–1591 of human TIAM1 (NP_003244.2), targeting a C-terminal region; this design may favor recognition of full-length and C-terminal isoforms.
• For Western blot (WB), consider optimizing antibody dilution (e.g., 1:500–1:2000) and loading adequate protein due to the large protein size (~178 kDa).
• In IHC-P, antigen retrieval and antibody titration are critical; validate performance in your tissue specimens.
• Suitable for ELISA applications, but appropriate coating and detection conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk monoclonals and polyclonals, and custom sourcing for rare targets. For researchers studying cell adhesion, migration, and Rho GTPase signaling, this TIAM1 antibody serves as a reliable tool, backed by our one-stop supply chain from concept to clinic.