Thyroid Peroxidase Rabbit Monoclonal Antibody for IHC-P/ELISA - P07202

Core Product Specifications and Parameters

Biological Background: Thyroid Peroxidase Function and Localization

• Thyroid peroxidase (TPO) is a key enzyme in thyroid hormone biosynthesis, catalyzing the iodination and coupling of hormonogenic tyrosines in thyroglobulin to produce the active hormones T3 and T4.
• TPO is a transmembrane glycoprotein located on the apical membrane of thyroid follicular cells, oriented with its enzymatic domain facing the follicular lumen.
• The protein contains a heme prosthetic group essential for its peroxidase activity, along with calcium and iron binding sites, disulfide bonds, and EGF-like domains that contribute to structural stability.
• Multiple splice variants of TPO have been identified, some of which may produce secreted or differently localized isoforms, though the full-length membrane-bound form is predominant.
• TPO is a major autoantigen in autoimmune thyroid diseases such as Hashimoto's thyroiditis and Graves' disease, and mutations in the TPO gene can cause congenital hypothyroidism.
• The UniProt record classifies TPO as a peroxidase, oxidoreductase, and thyroid hormone biosynthesis protein, with evidence at the protein level from proteomics identification.
• Post-translational modifications include N-glycosylation, which is important for proper folding and enzymatic activity.
• TPO expression is largely restricted to the thyroid gland, making it a specific marker for thyroid tissue in immunohistochemistry.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (amino acids 1-100) of human TPO, which may target an epitope conserved across human, mouse, and rat.
• For IHC-P, antigen retrieval methods such as heat-induced epitope retrieval in citrate buffer (pH 6.0) are often beneficial for membrane proteins; validate in your specific tissue type.
• In ELISA, use recombinant TPO or purified thyroglobulin fractions as coating antigen; consider blocking with non-fat milk or BSA to reduce background.
• Since the antibody is monoclonal, lot-to-lot consistency is expected; however, optimal working dilutions should be determined experimentally for each application and sample system.
• Cross-reactivity with mouse and rat has been confirmed, enabling use in rodent thyroid research models; verify by western blot or IHC on relevant tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a curated portfolio of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This Thyroid Peroxidase antibody supports thyroid function testing and autoimmune disease research, backed by CamelBio’s commitment to end-to-end supply chain reliability from concept to clinic.