Thymidylate synthase Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - P04818

Core Product Specifications and Parameters

Biological Background: Thymidylate synthase Function and Localization

• Thymidylate synthase (TYMS) is an essential enzyme in the de novo synthesis of thymidylate, a precursor for DNA replication and repair. Alternative splicing gives rise to multiple isoforms.
• The enzyme catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP) using 5,10-methylenetetrahydrofolate as a methyl donor.
• TYMS localizes to the nucleus, cytoplasm, and mitochondria, including the mitochondrial matrix and inner membrane, supporting thymidylate biosynthesis in multiple cellular compartments.
• As a phosphoprotein, its activity is regulated by phosphorylation. It also undergoes ubiquitylation and forms isopeptide bonds.
• Mutations in TYMS are associated with dyskeratosis congenita, a rare genetic disorder characterized by bone marrow failure and predisposition to cancer.
• Belongs to the thymidylate synthase family and functions as a methyltransferase, transferring a one-carbon unit to dUMP.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human TYMS protein (aa 1-313), which is expected to provide broad epitope coverage and robust reactivity across indicated species.
• Although validated for WB, IHC-P, IF/ICC, and ELISA, optimal working dilutions and conditions should be determined empirically in your experimental system.
• Given the mitochondrial and nuclear localization, consider appropriate subcellular fractionation or organelle staining controls for IF/ICC experiments.
• Cross-reactivity with mouse and rat has been reported, but sequence alignment may help predict performance in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This Thymidylate synthase Rabbit mAb supports nucleotide metabolism research and assay development. For rare or custom targets, our sourcing network can help bridge the gap from concept to clinic with efficient, one-stop procurement support.