THOC4/ALYREF Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q86V81

Core Product Specifications and Parameters

Biological Background: THOC4/ALYREF Function and Localization

• THOC4/ALYREF (also known as ALY, BEF, REF, THOC4) is the THO complex subunit 4, an mRNA export adapter and a key component of the TREX (transcription/export) complex, coupling transcription, processing, and nuclear export of spliced mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Related references: PMID:15833825, PMID:15998806, PMID:17190602
• The protein binds specifically to spliced mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner, facilitating mRNA export via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Related references: PMID:15833825, PMID:15998806, PMID:17190602
• THOC4/ALYREF also promotes the nuclear export of intronless mRNA, including HSP70 mRNA and m5C-containing mRNAs, by recognizing specific modifications and bridging interactions with DDX39B and NXF1-NXT1 (PubMed:17984224, PubMed:19165146, PubMed:28418038). Related references: PMID:17984224, PMID:19165146, PMID:28418038
• Beyond mRNA export, it acts as a chaperone that promotes dimerization of bZIP transcription factors, thereby enhancing transcriptional activation, and is involved in transcription elongation and genome stability (PubMed:10488337, PubMed:12438613). Related references: PMID:10488337, PMID:12438613
• The protein is predominantly localized to the nucleus and nuclear speckles but can also be found in the cytoplasm, reflecting its dynamic role in mRNP transport and metabolism.
• Post-translational modifications include acetylation, citrullination, methylation, and phosphorylation, suggesting regulatory mechanisms that may modulate its activity and interactions.
• THOC4/ALYREF is expressed in a wide variety of cancer types, indicating potential relevance in cancer biology and as a target for diagnostic or therapeutic applications.
• In infectious contexts, the TREX complex is hijacked for export of KSHV intronless mRNAs, and THOC4/ALYREF mediates recruitment of the complex to viral mRNAs, essential for virus production.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 158-257 of human THOC4/ALYREF. This region may generate an antibody that recognizes both human and rodent forms, as indicated by cross-reactivity with mouse and rat.
• For Western blot (WB), consider a starting dilution of 1:1000-1:5000 and validate in your sample system. Expect a band around 27 kDa.
• For immunohistochemistry (IHC-P), test on paraffin-embedded sections with appropriate antigen retrieval and validate staining specificity with positive and negative controls.
• For ELISA, optimize coating antigen and antibody concentration for best signal-to-noise ratio.
• The rabbit monoclonal format provides high consistency and reproducibility across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. This THOC4/ALYREF antibody, targeting a key mRNA export factor relevant in cancer and infectious disease research, is available alongside a wide range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. Let us help streamline your nucleic acid binding protein studies with reliable, ready-to-use reagents.