TGFB2 Rabbit pAb for WB, ELISA - P61812

Core Product Specifications and Parameters

Biological Background: TGFB2 Function and Localization

• TGFB2 encodes the transforming growth factor beta-2 proprotein, which is cleaved into two chains: Latency-associated peptide (LAP) and mature TGF-beta-2. The proprotein is also known as Cetermin and glioblastoma-derived T-cell suppressor factor (G-TSF).
• TGF-beta-2 is a multifunctional cytokine that regulates various processes, including angiogenesis and heart development. Related references: PMID:22772368, PMID:22772371
• The LAP chain maintains TGF-beta-2 in a latent state by non-covalently associating with the mature chain, and its activation is regulated by interactions with milieu molecules such as LTBP1 and LRRC32/GARP in the extracellular matrix.
• Following activation, mature TGF-beta-2 signals through binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce downstream signals.
• The protein is secreted and localizes to the extracellular space and extracellular matrix.
• Key post-translational modifications include cleavage on pairs of basic residues and glycosylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• Since the antibody is raised against a recombinant fragment spanning the proprotein region, it may detect both pro-TGFB2 and mature TGF-beta-2, depending on sample preparation and activation state. Consider reducing and non-reducing conditions in WB.
• For ELISA, validate the antibody’s reactivity with the recombinant immunogen and native protein in relevant biological samples (e.g., cell culture supernatants, serum).
• Cross-reactivity with mouse TGFB2 is reported; verify performance in mouse samples if used for cross-species studies.
• The antibody is polyclonal, so batch-to-batch consistency should be controlled for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, from validated antibody pairs to optimized polyclonal antibodies like this TGFB2 pAb, supporting your TGF-beta signaling research and diagnostic assay development. Our supply capabilities also include bulk ancillary reagents and custom sourcing for rare targets, ensuring seamless integration into your workflow.