TFPT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P0C1Z6

Core Product Specifications and Parameters

Biological Background: TFPT Function and Localization

• The human TFPT (TCF3 fusion partner) protein, also known as INO80 complex subunit F (INO80F) or protein FB1, is a regulatory component of the chromatin remodeling INO80 complex, which plays roles in transcriptional regulation, DNA replication, and DNA repair.
• TFPT is a nucleocytoplasmic protein that predominantly localizes to the nucleus, consistent with its involvement in chromatin remodeling and DNA maintenance.
• It promotes apoptosis in a p53/TP53-independent manner, suggesting a potential role in programmed cell death pathways distinct from classical tumor suppressor mechanisms.
• The INO80 complex, which includes TFPT, is involved in DNA damage response, recombination, and repair, highlighting its importance in genome stability.
• TFPT undergoes alternative splicing, producing multiple isoforms; it is also subject to phosphorylation and ubiquitination (isopeptide bond formation), as indicated by proteomics data.
• Classified as a proto-oncogene, TFPT has been implicated in chromosomal rearrangements, particularly in acute lymphoblastic leukemia (ALL) where it fuses with TCF3.
• Keywords include apoptosis, transcription regulation, DNA repair, and Ubl conjugation, underscoring its multifaceted role in cellular homeostasis.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of TFPT is approximately 28 kDa; however, post-translational modifications or isoform variation may result in additional bands. Consider using whole cell lysates from human cell lines known to express TFPT.
• For immunofluorescence/ICC, nuclear localization of TFPT should be observed. Use appropriate fixation and permeabilization methods, and validate with a nuclear counterstain.
• The ELISA application may be suitable for detecting TFPT in human samples; however, optimal conditions and detection limits should be empirically determined.
• Given the immunogen covers the N-terminal region (AA 1-253), this antibody may recognize multiple isoforms or processed forms, but cross-reactivity with other INO80 complex subunits should be assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to specialized IVD raw materials, including validated antibodies for apoptosis and chromatin research. Our portfolio includes TFPT and related INO80 complex antibodies, along with bulk ancillary reagents and customized sourcing solutions to accelerate your assay development. For rare targets and technical support, CamelBio offers a comprehensive one-stop supply bridge from concept to clinical application.