TFF1/pS2 Rabbit Monoclonal Antibody for WB, IP, IF-P, ELISA - P04155

Core Product Specifications and Parameters

Biological Background: TFF1/pS2 Function and Localization

• TFF1 (trefoil factor 1, also known as pS2, BCEI, HPS2, HP1.A, pNR-2) is a small secreted protein of 84 amino acids (9.15 kDa), belonging to the trefoil factor family.
• It functions as a stabilizer of the mucous gel overlying the gastrointestinal mucosa, providing a physical barrier against noxious agents.
• May also inhibit the growth of calcium oxalate crystals in urine.
• Secreted protein found in various mucosal surfaces, with highest expression in the upper gastric mucosal cells; also detected in goblet cells of the intestines, rectum, esophagus, sublingual gland, trachea, pancreatic ducts, gallbladder, vagina, and respiratory system.
• Strongly upregulated in breast cancer tissues and regulated by estrogen in MCF-7 cells, while normal mammary tissue shows low expression.
• In the gastrointestinal tract, highly expressed in normal gastric mucosa and regenerative tissues around ulcers, but expression is often lower in gastric cancer.
• Contains disulfide bonds and is classified as a growth factor; its 3D structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–84 of human TFF1/pS2, covering the mature secreted protein region; the antibody likely recognizes the native and denatured forms, suitable for Western blot (WB), immunoprecipitation (IP), and immunofluorescence (IF-P).
• For WB, consider using gastrointestinal tissue lysates or breast cancer cell lines (e.g., MCF-7) as positive controls; a band at approximately 9 kDa is expected under reducing conditions. Verify band specificity in your sample system.
• In IP and IF-P experiments, validate antibody performance with appropriate cellular or tissue models, and optimize fixation and permeabilization conditions for secreted proteins.
• The antibody cross-reacts with mouse and rat TFF1, but validate species-specific reactivity in your assay, particularly for ELISA applications.
• Since TFF1 is a secreted protein, ensure proper sample preparation to avoid loss during processing; consider analyzing concentrated culture supernatants or body fluids if applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality IVD raw materials, including this TFF1/pS2 rabbit monoclonal antibody and complementary reagents for gastrointestinal and cancer diagnostic research. With a broad portfolio covering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, CamelBio supports your assay development from concept to clinical application. Our sourcing capabilities extend to rare and custom raw materials, ensuring a seamless supply chain for your projects.